Matrix methods for solving protein substructures of chlorine and sulfur from anomalous data - PubMed (original) (raw)
. 2001 Dec;57(Pt 12):1857-62.
doi: 10.1107/s0907444901016535. Epub 2001 Nov 21.
Affiliations
- PMID: 11717499
- DOI: 10.1107/s0907444901016535
Matrix methods for solving protein substructures of chlorine and sulfur from anomalous data
R A de Graaff et al. Acta Crystallogr D Biol Crystallogr. 2001 Dec.
Abstract
The weak signal obtained from the anomalous scattering (at lambda = 1.54 A) of naturally occurring elements such as sulfur, phosphorus and ordered solvent chloride ions is used to determine the atomic positions of these atoms. Two examples are discussed: the sulfur and chlorine substructure of tetragonal hen egg-white lysozyme and an oligonucleotide containing ten P atoms. The substructure of lysozyme was also solved from Cu K(alpha) radiation data collected on a standard rotating-anode generator. The results presented here are an illustration of the power of the matrix methods, which are to be implemented in next distribution of the direct methods package CRUNCH.
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