The TolQ-TolR proteins energize TolA and share homologies with the flagellar motor proteins MotA-MotB - PubMed (original) (raw)
The TolQ-TolR proteins energize TolA and share homologies with the flagellar motor proteins MotA-MotB
E Cascales et al. Mol Microbiol. 2001 Nov.
Free article
Abstract
The Tol-Pal system of Escherichia coli is required for the maintenance of outer membrane stability. Recently, proton motive force (pmf) has been found to be necessary for the co-precipitation of the outer membrane lipoprotein Pal with the inner membrane TolA protein, indicating that the Tol-Pal system forms a transmembrane link in which TolA is energized. In this study, we show that both TolQ and TolR proteins are essential for the TolA-Pal interaction. A point mutation within the third transmembrane (TM) segment of TolQ was found to affect the TolA-Pal interaction strongly, whereas suppressor mutations within the TM segment of TolR restored this interaction. Modifying the Asp residue within the TM region of TolR indicated that an acidic residue was important for the pmf-dependent interaction of TolA with Pal and outer membrane stabilization. Analysis of sequence alignments of TolQ and TolR homologues from numerous Gram-negative bacterial genomes, together with analyses of the different tolQ-tolR mutants, revealed that the TM domains of TolQ and TolR present structural and functional homologies not only to ExbB and ExbD of the TonB system but also with MotA and MotB of the flagellar motor. The function of these three systems, as ion potential-driven molecular motors, is discussed
Similar articles
- Energy-dependent conformational change in the TolA protein of Escherichia coli involves its N-terminal domain, TolQ, and TolR.
Germon P, Ray MC, Vianney A, Lazzaroni JC. Germon P, et al. J Bacteriol. 2001 Jul;183(14):4110-4. doi: 10.1128/JB.183.14.4110-4114.2001. J Bacteriol. 2001. PMID: 11418549 Free PMC article. - Role of TolR N-terminal, central, and C-terminal domains in dimerization and interaction with TolA and tolQ.
Journet L, Rigal A, Lazdunski C, Bénédetti H. Journet L, et al. J Bacteriol. 1999 Aug;181(15):4476-84. doi: 10.1128/JB.181.15.4476-4484.1999. J Bacteriol. 1999. PMID: 10419942 Free PMC article. - The Tol proteins of Escherichia coli and their involvement in the uptake of biomolecules and outer membrane stability.
Lazzaroni JC, Germon P, Ray MC, Vianney A. Lazzaroni JC, et al. FEMS Microbiol Lett. 1999 Aug 15;177(2):191-7. doi: 10.1111/j.1574-6968.1999.tb13731.x. FEMS Microbiol Lett. 1999. PMID: 10474183 Review. - The Tol-Pal proteins of the Escherichia coli cell envelope: an energized system required for outer membrane integrity?
Lloubès R, Cascales E, Walburger A, Bouveret E, Lazdunski C, Bernadac A, Journet L. Lloubès R, et al. Res Microbiol. 2001 Jul-Aug;152(6):523-9. doi: 10.1016/s0923-2508(01)01226-8. Res Microbiol. 2001. PMID: 11501670 Review.
Cited by
- Evolution of ceftazidime-avibactam and cefiderocol resistance in ST131-H30R1-Escherichia coli isolates with KPC-3 mutants and application of FTIR biotyping.
Castillo-Polo JA, Hernández-García M, Maruri-Aransolo A, de la Vega C, Ruiz-Garbajosa P, Cantón R. Castillo-Polo JA, et al. Microbiol Spectr. 2024 Apr 2;12(4):e0277623. doi: 10.1128/spectrum.02776-23. Epub 2024 Feb 28. Microbiol Spectr. 2024. PMID: 38415657 Free PMC article. - Tunable force transduction through the Escherichia coli cell envelope.
Williams-Jones DP, Webby MN, Press CE, Gradon JM, Armstrong SR, Szczepaniak J, Kleanthous C. Williams-Jones DP, et al. Proc Natl Acad Sci U S A. 2023 Nov 21;120(47):e2306707120. doi: 10.1073/pnas.2306707120. Epub 2023 Nov 16. Proc Natl Acad Sci U S A. 2023. PMID: 37972066 Free PMC article. - Critical role of the RpoE stress response pathway in polymyxin resistance of Escherichia coli.
Zeng X, Hinenoya A, Guan Z, Xu F, Lin J. Zeng X, et al. J Antimicrob Chemother. 2023 Mar 2;78(3):732-746. doi: 10.1093/jac/dkad003. J Antimicrob Chemother. 2023. PMID: 36658759 Free PMC article. - The Ton Motor.
Ratliff AC, Buchanan SK, Celia H. Ratliff AC, et al. Front Microbiol. 2022 Apr 7;13:852955. doi: 10.3389/fmicb.2022.852955. eCollection 2022. Front Microbiol. 2022. PMID: 35464957 Free PMC article. Review. - Roles of the Tol/Pal System in Bacterial Pathogenesis and Its Application to Antibacterial Therapy.
Hirakawa H, Suzue K, Tomita H. Hirakawa H, et al. Vaccines (Basel). 2022 Mar 10;10(3):422. doi: 10.3390/vaccines10030422. Vaccines (Basel). 2022. PMID: 35335056 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases