Two-step transcriptional amplification as a method for imaging reporter gene expression using weak promoters - PubMed (original) (raw)

(A) In vivo optical CCD imaging of mice carrying transiently transfected LNCaP cells for control studies. All images shown are the visible light image superimposed on the optical CCD image with a scale in RLU/min as shown. (Panel 1) LNCaP cells were transiently transfected with only the L5 vector (control) and cells implanted i.p. The animal was then imaged after injection of

-luciferin (i.p.). (Panel 2) The same animal as in Panel 1 was imaged 48 h after implantation of androgen pellet by readministering

-luciferin (i.p.). (Panel 3) LNCaP cells were transiently transfected with the PG and L5 vectors and cells implanted i.p. The mouse was imaged after i.p. injection of

-luciferin. (Panel 4) The same animal imaged in Panel 3 was reimaged in the absence of androgen 48 h later after i.p. injection of

-luciferin. (B) In vivo optical CCD imaging of mice carrying transiently transfected LNCaP cells for comparison of one-step and TSTA. All images shown are the visible light image superimposed on the optical CCD image with a scale in RLU/min as shown. (Panel 1) LNCaP cells were transiently transfected with only the one-step PL vector and cells implanted i.p. The animal was then imaged after injection of

-luciferin (i.p.). (Panel 2) The same mouse in Panel 1 was reimaged with i.p. injection of

-luciferin 48 h after implantation of an androgen pellet. (Panel 3) LNCaP cells were transiently transfected with the PG and L5 vectors (TSTA system) and cells implanted i.p. The mouse was then imaged after i.p. injection of

-luciferin. (Panel 4) The same animal imaged in Panel 3 was reimaged with i.p.

-luciferin 48 h after implantation of an androgen pellet.