Sequence of the 50-kb conjugative multiresistance plasmid pRE25 from Enterococcus faecalis RE25 - PubMed (original) (raw)
Comparative Study
Sequence of the 50-kb conjugative multiresistance plasmid pRE25 from Enterococcus faecalis RE25
F V Schwarz et al. Plasmid. 2001 Nov.
Abstract
The complete 50,237-bp DNA sequence of the conjugative and mobilizing multiresistance plasmid pRE25 from Enterococcus faecalis RE25 was determined. The plasmid had 58 putative open reading frames, 5 of which encode resistance to 12 antimicrobials. Chloramphenicol acetyltransferase and the 23S RNA methylase are identical to gene products of the broad-host-range plasmid pIP501 from Streptococcus agalactiae. In addition, a 30.5-kb segment is almost identical to pIP501. Genes encoding an aminoglycoside 6-adenylyltransferase, a streptothricin acetyltransferase, and an aminoglycoside phosphotransferase are arranged in tandem on a 7.4-kb fragment as previously reported in Tn5405 from Staphylococcus aureus and in pJH1 from E. faecalis. One interrupted and five complete IS elements as well as three replication genes were also identified. pRE25 was transferred by conjugation to E. faecalis, Listeria innocua, and Lactococcus lactis by means of a transfer region that appears similar to that of pIP501. It is concluded that pRE25 may contribute to the further spread of antibiotic-resistant microorganisms via food into the human community.
Copyright 2001 Elsevier Science.
Similar articles
- Molecular structure and evolution of the conjugative multiresistance plasmid pRE25 of Enterococcus faecalis isolated from a raw-fermented sausage.
Teuber M, Schwarz F, Perreten V. Teuber M, et al. Int J Food Microbiol. 2003 Dec 1;88(2-3):325-9. doi: 10.1016/s0168-1605(03)00195-8. Int J Food Microbiol. 2003. PMID: 14597005 Review. - Construction and characterization of Enterococcus faecalis CG110/gfp/pRE25*, a tool for monitoring horizontal gene transfer in complex microbial ecosystems.
Haug MC, Tanner SA, Lacroix C, Meile L, Stevens MJ. Haug MC, et al. FEMS Microbiol Lett. 2010 Dec;313(2):111-9. doi: 10.1111/j.1574-6968.2010.02131.x. Epub 2010 Oct 28. FEMS Microbiol Lett. 2010. PMID: 21029153 - Conjugative transfer of the Lactococcus lactis sex factor and pRS01 plasmid to Enterococcus faecalis.
Belhocine K, Mandilaras V, Yeung B, Cousineau B. Belhocine K, et al. FEMS Microbiol Lett. 2007 Apr;269(2):289-94. doi: 10.1111/j.1574-6968.2007.00641.x. Epub 2007 Jan 30. FEMS Microbiol Lett. 2007. PMID: 17263841 - The conjugative transposon Tn916 of Enterococcus faecalis: structural analysis and some key factors involved in movement.
Clewell DB, Jaworski DD, Flannagan SE, Zitzow LA, Su YA. Clewell DB, et al. Dev Biol Stand. 1995;85:11-7. Dev Biol Stand. 1995. PMID: 8586160 Review. No abstract available.
Cited by
- Complete genome sequence of Enterococcus faecium strain TX16 and comparative genomic analysis of Enterococcus faecium genomes.
Qin X, Galloway-Peña JR, Sillanpaa J, Roh JH, Nallapareddy SR, Chowdhury S, Bourgogne A, Choudhury T, Muzny DM, Buhay CJ, Ding Y, Dugan-Rocha S, Liu W, Kovar C, Sodergren E, Highlander S, Petrosino JF, Worley KC, Gibbs RA, Weinstock GM, Murray BE. Qin X, et al. BMC Microbiol. 2012 Jul 7;12:135. doi: 10.1186/1471-2180-12-135. BMC Microbiol. 2012. PMID: 22769602 Free PMC article. - Functional identification of conjugation and replication regions of the tetracycline resistance plasmid pCW3 from Clostridium perfringens.
Bannam TL, Teng WL, Bulach D, Lyras D, Rood JI. Bannam TL, et al. J Bacteriol. 2006 Jul;188(13):4942-51. doi: 10.1128/JB.00298-06. J Bacteriol. 2006. PMID: 16788202 Free PMC article. - Plasmid-Borne erm(T) from invasive, macrolide-resistant Streptococcus pyogenes strains.
Woodbury RL, Klammer KA, Xiong Y, Bailiff T, Glennen A, Bartkus JM, Lynfield R, Van Beneden C, Beall BW; Active Bacterial Core Surveillance Team. Woodbury RL, et al. Antimicrob Agents Chemother. 2008 Mar;52(3):1140-3. doi: 10.1128/AAC.01352-07. Epub 2008 Jan 7. Antimicrob Agents Chemother. 2008. PMID: 18180360 Free PMC article. - The ClpXP protease is responsible for the degradation of the Epsilon antidote to the Zeta toxin of the streptococcal pSM19035 plasmid.
Brzozowska I, Zielenkiewicz U. Brzozowska I, et al. J Biol Chem. 2014 Mar 14;289(11):7514-23. doi: 10.1074/jbc.M113.519488. Epub 2014 Feb 3. J Biol Chem. 2014. PMID: 24492616 Free PMC article. - Vancomycin Resistance in Enterococcus and Staphylococcus aureus.
Li G, Walker MJ, De Oliveira DMP. Li G, et al. Microorganisms. 2022 Dec 21;11(1):24. doi: 10.3390/microorganisms11010024. Microorganisms. 2022. PMID: 36677316 Free PMC article. Review.