Bacteroides ovatus as the predominant commensal intestinal microbe causing a systemic antibody response in inflammatory bowel disease - PubMed (original) (raw)

Bacteroides ovatus as the predominant commensal intestinal microbe causing a systemic antibody response in inflammatory bowel disease

Shin Saitoh et al. Clin Diagn Lab Immunol. 2002 Jan.

Abstract

To clarify what bacterial species of commensal intestinal microbes are recognized as the antigens that induce a serum antibody response in patients with inflammatory bowel disease (IBD), 72 subjects consisting of 12 Crohn's disease patients, 30 ulcerative colitis patients, and 30 healthy volunteers were examined for their titers of serum antibody to these intestinal bacteria. In IBD patients, as a result, significant elevations of both the immunoglobulin G (IgG) and IgA titers to Bacteroides ovatus were found. Immunoblotting showed that a definite 19.5-kDa band of B. ovatus was bound to the serum antibody raised in IBD patients. It was thus concluded that B. ovatus causes serum antibody responses in IBD patients, and a 19.5-kDa molecule of this bacterium appears to be the responsible antigen, although the role of this event in pathogenesis remains unclear.

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Figures

FIG. 1.

FIG. 1.

Counts of fecal bacteria in the bacterial group and levels of serum antibody to the bacteria. The number of bacteria belonging to each bacterial group in the feces (open bars) and the autologous titer of serum antibody to the bacterial species (filled bars) which is most frequently isolated in each bacterial group were measured in CD-1, CD-2, CD-3, and HV-1 by the method discussed in Materials and Methods. N.D., not detected. Δ FI, change in fluorescence intensity.

FIG. 2.

FIG. 2.

Measurement of the serum antibody titer to Bacteroides by ELISA in the IBD populations. Titers of IgG and IgA class antibodies reacting with Bacteroides strain TS2 (A), B. vulgatus JCM 5826 (B), and B. ovatus JCM 5824 (C) were measured by ELISA in the sera of HV, UC, and CD subjects. Each dot represents the mean value for each subject from triplicate samples. A horizontal bar with a number on its right represents the mean value for each population. NS, not significant.

FIG. 2.

FIG. 2.

Measurement of the serum antibody titer to Bacteroides by ELISA in the IBD populations. Titers of IgG and IgA class antibodies reacting with Bacteroides strain TS2 (A), B. vulgatus JCM 5826 (B), and B. ovatus JCM 5824 (C) were measured by ELISA in the sera of HV, UC, and CD subjects. Each dot represents the mean value for each subject from triplicate samples. A horizontal bar with a number on its right represents the mean value for each population. NS, not significant.

FIG. 2.

FIG. 2.

Measurement of the serum antibody titer to Bacteroides by ELISA in the IBD populations. Titers of IgG and IgA class antibodies reacting with Bacteroides strain TS2 (A), B. vulgatus JCM 5826 (B), and B. ovatus JCM 5824 (C) were measured by ELISA in the sera of HV, UC, and CD subjects. Each dot represents the mean value for each subject from triplicate samples. A horizontal bar with a number on its right represents the mean value for each population. NS, not significant.

FIG. 3.

FIG. 3.

Immunoblotting using whole bacterial lysates and the serum from an IBD patient. The whole bacterial lysate obtained from TS2, B. ovatus JCM 5824, B. vulgatus JCM 5826, or E. coli JCM 1649 was loaded in each lane as shown at the top. In the two lanes on the far left, the lysates were treated by 2-ME before being loaded onto the gel. The numbers indicated in the left margin of the gel represent the molecular masses (in thousands) as determined by the mobilties of the molecular mass markers. The serum from a CD patient (patient 2) was used as an antibody for the reaction.

FIG. 4.

FIG. 4.

Treatment of serum by absorption with whole bacterial cells before being used in immunoblotting. Serum from patient 2 was treated by absorption using whole bacterial cells of L. salivarius (L.s) or B. ovatus (B.o) as indicated at the top of the figure. The serum without such treatment was used as a control (None). Then the serum was reacted with a filter to which whole bacterial lysates from L. salivarius, B. ovatus, and B. vulgatus (B.v) were loaded as indicated at the bottom of the figure.

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