Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis - PubMed (original) (raw)

Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis

K Murray et al. Biochem J. 1976 Nov.

Abstract

Two endonucleases, AvaI and AvaII, were isolated from Anabaena variabilis on the basis of their ability to make a limited number of breaks at specific points in bacteriophage lambda DNA. Neither enzyme has cofactor requirements beyond Mg2+. Endonuclease AvaI makes eight breaks in the phage lambda chromosome at which the 5'-terminal sequence is pPy-C-G-N. AvaII endonuclease cuts phage lambda DNA more extensively, yielding fragments with the 5'-terminal sequence G-T-C-N or G-A-C-N. Neither enzyme generates cohesive ends.

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