Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein - PubMed (original) (raw)

. 2002 Apr 5;277(14):12190-9.

doi: 10.1074/jbc.M108665200. Epub 2002 Jan 10.

Affiliations

Free article

Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein

Andrew J Lindsay et al. J Biol Chem. 2002.

Free article

Abstract

Rab4 and Rab11 are small GTPases belonging to the Ras superfamily. They both function as regulators along the receptor recycling pathway. We have identified a novel 80-kDa protein that interacts specifically with the GTP-bound conformation of Rab4, and subsequent work has shown that it also interacts strongly with Rab11. We name this protein Rab coupling protein (RCP). RCP is predominantly membrane-bound and is expressed in all cell lines and tissues tested. It colocalizes with early endosomal markers including Rab4 and Rab11 as well as with the transferrin receptor. Overexpression of the carboxyl-terminal region of RCP, which contains the Rab4- and Rab11-interacting domain, results in a dramatic tubulation of the transferrin compartment. Furthermore, expression of this mutant causes a significant reduction in endosomal recycling without affecting ligand uptake or degradation in quantitative assays. RCP is a homologue of Rip11 and therefore belongs to the recently described Rab11-FIP family.

PubMed Disclaimer

Similar articles

Cited by

Publication types

MeSH terms

Substances

LinkOut - more resources