Localization and functional relevance of system a neutral amino acid transporters in cultured hippocampal neurons - PubMed (original) (raw)
. 2002 Mar 22;277(12):10467-73.
doi: 10.1074/jbc.M110942200. Epub 2002 Jan 11.
Affiliations
- PMID: 11788594
- DOI: 10.1074/jbc.M110942200
Free article
Localization and functional relevance of system a neutral amino acid transporters in cultured hippocampal neurons
Simona Armano et al. J Biol Chem. 2002.
Free article
Abstract
Glutamine and alanine are important precursors for the synthesis of glutamate. Provided to neurons by neighboring astrocytes, these amino acids are internalized by classical system A amino acid carriers. In particular, System A transporter (SAT1) is a highly efficient glutamine transporter, whereas SAT2 exhibits broad specificity for neutral amino acids with a preference for alanine. We investigated the localization and the functional relevance of SAT1 and SAT2 in primary cultures of hippocampal neurons. Both carriers have been expressed since early developmental stages and are uniformly distributed throughout all neuronal processes. However, whereas SAT1 is present in axonal growth cones and can be detected at later developmental stages at the sites of synaptic contacts, SAT2 does not appear to be significantly expressed in these compartments. The non-metabolizable amino acid analogue alpha-(methylamino)-isobutyric acid, a competitive inhibitor of system A carriers, significantly reduced miniature excitatory postsynaptic current amplitude in neurons growing on top of astrocytes, being ineffective in pure neuronal cultures. alpha-(Methylamino)-isobutyric acid did not alter neuronal responsitivity to glutamate, thus excluding a postsynaptic effect. These data indicate that system A carriers are expressed with a different subcellular distribution in hippocampal neurons and play a crucial role in controlling the astrocyte-mediated supply of glutamatergic neurons with neurotransmitter precursors.
Similar articles
- System A transporter SAT2 mediates replenishment of dendritic glutamate pools controlling retrograde signaling by glutamate.
Jenstad M, Quazi AZ, Zilberter M, Haglerød C, Berghuis P, Saddique N, Goiny M, Buntup D, Davanger S, S Haug FM, Barnes CA, McNaughton BL, Ottersen OP, Storm-Mathisen J, Harkany T, Chaudhry FA. Jenstad M, et al. Cereb Cortex. 2009 May;19(5):1092-106. doi: 10.1093/cercor/bhn151. Epub 2008 Oct 1. Cereb Cortex. 2009. PMID: 18832333 - Glutamine uptake by System A transporters maintains neurotransmitter GABA synthesis and inhibitory synaptic transmission.
Fricke MN, Jones-Davis DM, Mathews GC. Fricke MN, et al. J Neurochem. 2007 Sep;102(6):1895-1904. doi: 10.1111/j.1471-4159.2007.04649.x. Epub 2007 May 14. J Neurochem. 2007. PMID: 17504265 - Functional expression of two system A glutamine transporter isoforms in rat auditory brainstem neurons.
Blot A, Billups D, Bjørkmo M, Quazi AZ, Uwechue NM, Chaudhry FA, Billups B. Blot A, et al. Neuroscience. 2009 Dec 15;164(3):998-1008. doi: 10.1016/j.neuroscience.2009.09.015. Epub 2009 Sep 12. Neuroscience. 2009. PMID: 19751803 Free PMC article. - Roles of glutamine in neurotransmission.
Albrecht J, Sidoryk-Węgrzynowicz M, Zielińska M, Aschner M. Albrecht J, et al. Neuron Glia Biol. 2010 Nov;6(4):263-76. doi: 10.1017/S1740925X11000093. Epub 2011 Oct 21. Neuron Glia Biol. 2010. PMID: 22018046 Review. - Brain glutamate metabolism: neuronal-astroglial relationships.
Yudkoff M, Nissim I, Daikhin Y, Lin ZP, Nelson D, Pleasure D, Erecinska M. Yudkoff M, et al. Dev Neurosci. 1993;15(3-5):343-50. doi: 10.1159/000111354. Dev Neurosci. 1993. PMID: 7805588 Review.
Cited by
- SAT1, A Glutamine Transporter, is Preferentially Expressed in GABAergic Neurons.
Solbu TT, Bjørkmo M, Berghuis P, Harkany T, Chaudhry FA. Solbu TT, et al. Front Neuroanat. 2010 Feb 8;4:1. doi: 10.3389/neuro.05.001.2010. eCollection 2010. Front Neuroanat. 2010. PMID: 20161990 Free PMC article. - The SLC38 family of sodium-amino acid co-transporters.
Bröer S. Bröer S. Pflugers Arch. 2014 Jan;466(1):155-72. doi: 10.1007/s00424-013-1393-y. Epub 2013 Nov 6. Pflugers Arch. 2014. PMID: 24193407 Review. - Impact of Inhibition of Glutamine and Alanine Transport on Cerebellar Glial and Neuronal Metabolism.
Das A, Gauthier-Coles G, Bröer S, Rae CD. Das A, et al. Biomolecules. 2022 Aug 27;12(9):1189. doi: 10.3390/biom12091189. Biomolecules. 2022. PMID: 36139028 Free PMC article. - Sodium-coupled neutral amino acid (System N/A) transporters of the SLC38 gene family.
Mackenzie B, Erickson JD. Mackenzie B, et al. Pflugers Arch. 2004 Feb;447(5):784-95. doi: 10.1007/s00424-003-1117-9. Epub 2003 Jul 4. Pflugers Arch. 2004. PMID: 12845534 Review. - Protein kinase C-mediated phosphorylation of a single serine residue on the rat glial glutamine transporter SN1 governs its membrane trafficking.
Nissen-Meyer LS, Popescu MC, Hamdani el H, Chaudhry FA. Nissen-Meyer LS, et al. J Neurosci. 2011 Apr 27;31(17):6565-75. doi: 10.1523/JNEUROSCI.3694-10.2011. J Neurosci. 2011. PMID: 21525297 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials