Global expression profiling of acetate-grown Escherichia coli - PubMed (original) (raw)
. 2002 Apr 12;277(15):13175-83.
doi: 10.1074/jbc.M110809200. Epub 2002 Jan 28.
Affiliations
- PMID: 11815613
- DOI: 10.1074/jbc.M110809200
Free article
Global expression profiling of acetate-grown Escherichia coli
Min-Kyu Oh et al. J Biol Chem. 2002.
Free article
Abstract
This study characterized the transcript profile of Escherichia coli in acetate cultures using DNA microarray on glass slides. Glucose-grown cultures were used as a reference. At the 95% confidence level, 354 genes were up-regulated in acetate, while 370 genes were down-regulated compared with the glucose-grown culture. Generally, more metabolic genes were up-regulated in acetate than other gene groups, while genes involved in cell replication, transcription, and translation machinery tended to be down-regulated. It appears that E. coli commits more resources to metabolism at the expense of growth when cultured in the poor carbon source. The expression profile confirms many known features in acetate metabolism such as the induction of the glyoxylate pathway, tricarboxylic acid cycle, and gluconeogenic genes. It also provided many previously unknown features, including induction of malic enzymes, ppsA, and the glycolate pathway and repression of glycolytic and glucose phosphotransferase genes in acetate. The carbon flux delivered from the malic enzymes and PpsA in acetate was further confirmed by deletion mutations. In general, the gene expression profiles qualitatively agree with the metabolic flux changes and may serve as a predictor for gene function and metabolic flux distribution.
Similar articles
- A global regulatory role of gluconeogenic genes in Escherichia coli revealed by transcriptome network analysis.
Kao KC, Tran LM, Liao JC. Kao KC, et al. J Biol Chem. 2005 Oct 28;280(43):36079-87. doi: 10.1074/jbc.M508202200. Epub 2005 Aug 31. J Biol Chem. 2005. PMID: 16141204 - Gene expression profiling by DNA microarrays and metabolic fluxes in Escherichia coli.
Oh MK, Liao JC. Oh MK, et al. Biotechnol Prog. 2000 Mar-Apr;16(2):278-86. doi: 10.1021/bp000002n. Biotechnol Prog. 2000. PMID: 10753455 - Transcriptome analysis of acetate metabolism in Corynebacterium glutamicum using a newly developed metabolic array.
Hayashi M, Mizoguchi H, Shiraishi N, Obayashi M, Nakagawa S, Imai J, Watanabe S, Ota T, Ikeda M. Hayashi M, et al. Biosci Biotechnol Biochem. 2002 Jun;66(6):1337-44. doi: 10.1271/bbb.66.1337. Biosci Biotechnol Biochem. 2002. PMID: 12162556 - Utilization of acetate in Escherichia coli: structural organization and differential expression of the ace operon.
Cortay JC, Bleicher F, Duclos B, Cenatiempo Y, Gautier C, Prato JL, Cozzone AJ. Cortay JC, et al. Biochimie. 1989 Sep-Oct;71(9-10):1043-9. doi: 10.1016/0300-9084(89)90109-0. Biochimie. 1989. PMID: 2512996 Review. - Genome-wide transcription profiling of Corynebacterium glutamicum after heat shock and during growth on acetate and glucose.
Muffler A, Bettermann S, Haushalter M, Hörlein A, Neveling U, Schramm M, Sorgenfrei O. Muffler A, et al. J Biotechnol. 2002 Sep 25;98(2-3):255-68. doi: 10.1016/s0168-1656(02)00136-0. J Biotechnol. 2002. PMID: 12141991 Review.
Cited by
- Enhancing metabolic efficiency via novel constitutive promoters to produce protocatechuic acid in Escherichia coli.
Örn OE, Hagman A, Ismail M, Leiva Eriksson N, Hatti-Kaul R. Örn OE, et al. Appl Microbiol Biotechnol. 2024 Aug 17;108(1):442. doi: 10.1007/s00253-024-13256-6. Appl Microbiol Biotechnol. 2024. PMID: 39153079 Free PMC article. - Integrative in vivo analysis of the ethanolamine utilization bacterial microcompartment in Escherichia coli.
Jallet D, Soldan V, Shayan R, Stella A, Ismail N, Zenati R, Cahoreau E, Burlet-Schiltz O, Balor S, Millard P, Heux S. Jallet D, et al. mSystems. 2024 Aug 20;9(8):e0075024. doi: 10.1128/msystems.00750-24. Epub 2024 Jul 18. mSystems. 2024. PMID: 39023255 Free PMC article. - Effect of adaptive laboratory evolution of engineered Escherichia coli in acetate on the biosynthesis of succinic acid from glucose in two-stage cultivation.
Jiang J, Luo Y, Fei P, Zhu Z, Peng J, Lu J, Zhu D, Wu H. Jiang J, et al. Bioresour Bioprocess. 2024 Apr 5;11(1):34. doi: 10.1186/s40643-024-00749-5. Bioresour Bioprocess. 2024. PMID: 38647614 Free PMC article. - Efficient natural plasmid transformation of Vibrio natriegens enables zero-capital molecular biology.
Specht DA, Sheppard TJ, Kennedy F, Li S, Gadikota G, Barstow B. Specht DA, et al. PNAS Nexus. 2024 Feb 13;3(2):pgad444. doi: 10.1093/pnasnexus/pgad444. eCollection 2024 Feb. PNAS Nexus. 2024. PMID: 38352175 Free PMC article. - Transcriptional and translational flux optimization at the key regulatory node for enhanced production of naringenin using acetate in engineered Escherichia coli.
Kim DH, Hwang HG, Ye DY, Jung GY. Kim DH, et al. J Ind Microbiol Biotechnol. 2024 Jan 9;51:kuae006. doi: 10.1093/jimb/kuae006. J Ind Microbiol Biotechnol. 2024. PMID: 38285614 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases