Visualization and quantification of T cell-mediated cytotoxicity using cell-permeable fluorogenic caspase substrates - PubMed (original) (raw)

Visualization and quantification of T cell-mediated cytotoxicity using cell-permeable fluorogenic caspase substrates

Luzheng Liu et al. Nat Med. 2002 Feb.

Abstract

We have developed a non-radioactive flow-cytometry assay to monitor and quantify the target-cell killing activities mediated by cytotoxic T lymphocytes (CTLs). This flow-cytometry CTL (FCC) assay is predicated on measurement of CTL-induced caspase activation in target cells through detection of the specific cleavage of fluorogenic caspase substrates. Here we show that this assay reliably detects antigen-specific CTL killing of target cells, and demonstrate that it provides a more sensitive, more informative and safer alternative to the standard 51Cr-release assay most often used to quantify CTL responses. The FCC assay can be used to study CTL-mediated killing of primary host target cells of different cell lineages, and enables the study of antigen-specific cellular immune responses in real time at the single-cell level. As such, the FCC assay can provide a valuable tool for studies of infectious disease pathogenesis and development of new vaccines and immunotherapies.

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Measuring T-cell-mediated cytotoxicity using antibody to activated caspase 3.
Jerome KR, Sloan DD, Aubert M. Jerome KR, et al. Nat Med. 2003 Jan;9(1):4-5. doi: 10.1038/nm0103-4. Nat Med. 2003. PMID: 12514701 No abstract available.

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Visualization and quantification of T cell-mediated cytotoxicity using cell-permeable fluorogenic caspase substrates - PubMed (original) (raw)