Requirement of the activation-induced deaminase (AID) gene for immunoglobulin gene conversion - PubMed (original) (raw)

. 2002 Feb 15;295(5558):1301-6.

doi: 10.1126/science.1067308.

Affiliations

• PMID: 11847344
• DOI: 10.1126/science.1067308

Requirement of the activation-induced deaminase (AID) gene for immunoglobulin gene conversion

Hiroshi Arakawa et al. Science. 2002.

Abstract

Three phenotypically distinct processes-somatic hypermutation, gene conversion, and switch recombination-remodel the functionally rearranged immunoglobulin (Ig) loci in B cells. Somatic hypermutation and switch recombination have recently been shown to depend on the activation-induced deaminase (AID) gene product. Here, we show that the disruption of the AID gene in the chicken B cell line DT40 completely blocks Ig gene conversion and that this block can be complemented by reintroduction of the AID complementary DNA. This demonstrates that the AID master gene controls all B cell-specific modifications of vertebrate Ig genes.

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