Beta-adrenoceptor-mediated control of apical membrane conductive properties in fetal distal lung epithelia - PubMed (original) (raw)
. 2002 Apr;282(4):L621-30.
doi: 10.1152/ajplung.00142.2001.
Affiliations
- PMID: 11880286
- DOI: 10.1152/ajplung.00142.2001
Free article
Beta-adrenoceptor-mediated control of apical membrane conductive properties in fetal distal lung epithelia
A Collett et al. Am J Physiol Lung Cell Mol Physiol. 2002 Apr.
Free article
Abstract
Distal lung epithelial cells isolated from fetal rats were cultured (48 h) on permeable supports so that transepithelial ion transport could be quantified electrometrically. Unstimulated cells generated a short-circuit current (I(sc)) that was inhibited (~80%) by apical amiloride. The current is thus due, predominantly, to the absorption of Na(+) from the apical solution. Isoprenaline increased the amiloride-sensitive I(sc) about twofold. Experiments in which apical membrane Na(+) currents were monitored in basolaterally permeabilized cells showed that this was accompanied by a rise in apical Na(+) conductance (G(Na(+))). Isoprenaline also increased apical Cl- conductance (G(Cl-)) by activating an anion channel species sensitive to glibenclamide but unaffected by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). The isoprenaline-evoked changes in G(Na(+)) and G(Cl(minus sign)) could account for the changes in I(sc) observed in intact cells. Glibenclamide had no effect upon the isoprenaline-evoked stimulation of I(sc) or G(Na(+)) demonstrating that the rise in G(Cl-) is not essential to the stimulation of Na(+) transport.
Similar articles
- cAMP regulation of Cl(-) and HCO(-)(3) secretion across rat fetal distal lung epithelial cells.
Lazrak A, Thome U, Myles C, Ware J, Chen L, Venglarik CJ, Matalon S. Lazrak A, et al. Am J Physiol Lung Cell Mol Physiol. 2002 Apr;282(4):L650-8. doi: 10.1152/ajplung.00370.2001. Am J Physiol Lung Cell Mol Physiol. 2002. PMID: 11880289 - Hormonal modulation of Na(+) transport in rat fetal distal lung epithelial cells.
Ramminger SJ, Inglis SK, Olver RE, Wilson SM. Ramminger SJ, et al. J Physiol. 2002 Oct 15;544(2):567-77. doi: 10.1113/jphysiol.2002.022459. J Physiol. 2002. PMID: 12381827 Free PMC article. - Cl-channel activation is necessary for stimulation of Na transport in adult alveolar epithelial cells.
O'Grady SM, Jiang X, Ingbar DH. O'Grady SM, et al. Am J Physiol Lung Cell Mol Physiol. 2000 Feb;278(2):L239-44. doi: 10.1152/ajplung.2000.278.2.L239. Am J Physiol Lung Cell Mol Physiol. 2000. PMID: 10666106 Review. - Mechanisms of increased Na(+) transport in ATII cells by cAMP: we agree to disagree and do more experiments.
Lazrak A, Nielsen VG, Matalon S. Lazrak A, et al. Am J Physiol Lung Cell Mol Physiol. 2000 Feb;278(2):L233-8. doi: 10.1152/ajplung.2000.278.2.L233. Am J Physiol Lung Cell Mol Physiol. 2000. PMID: 10666105 Review.
Cited by
- δ ENaC: a novel divergent amiloride-inhibitable sodium channel.
Ji HL, Zhao RZ, Chen ZX, Shetty S, Idell S, Matalon S. Ji HL, et al. Am J Physiol Lung Cell Mol Physiol. 2012 Dec 15;303(12):L1013-26. doi: 10.1152/ajplung.00206.2012. Epub 2012 Sep 14. Am J Physiol Lung Cell Mol Physiol. 2012. PMID: 22983350 Free PMC article. Review. - AMP-activated protein kinase (AMPK)-dependent and -independent pathways regulate hypoxic inhibition of transepithelial Na+ transport across human airway epithelial cells.
Tan CD, Smolenski RT, Harhun MI, Patel HK, Ahmed SG, Wanisch K, Yáñez-Muñoz RJ, Baines DL. Tan CD, et al. Br J Pharmacol. 2012 Sep;167(2):368-82. doi: 10.1111/j.1476-5381.2012.01993.x. Br J Pharmacol. 2012. PMID: 22509822 Free PMC article. - SGK1 activity in Na+ absorbing airway epithelial cells monitored by assaying NDRG1-Thr346/356/366 phosphorylation.
Inglis SK, Gallacher M, Brown SG, McTavish N, Getty J, Husband EM, Murray JT, Wilson SM. Inglis SK, et al. Pflugers Arch. 2009 Apr;457(6):1287-301. doi: 10.1007/s00424-008-0587-1. Epub 2008 Sep 12. Pflugers Arch. 2009. PMID: 18787837 - AICAR decreases the activity of two distinct amiloride-sensitive Na+-permeable channels in H441 human lung epithelial cell monolayers.
Albert AP, Woollhead AM, Mace OJ, Baines DL. Albert AP, et al. Am J Physiol Lung Cell Mol Physiol. 2008 Nov;295(5):L837-48. doi: 10.1152/ajplung.90353.2008. Epub 2008 Aug 22. Am J Physiol Lung Cell Mol Physiol. 2008. PMID: 18723760 Free PMC article. - AICAR activates AMPK and alters PIP2 association with the epithelial sodium channel ENaC to inhibit Na+ transport in H441 lung epithelial cells.
Mace OJ, Woollhead AM, Baines DL. Mace OJ, et al. J Physiol. 2008 Sep 15;586(18):4541-57. doi: 10.1113/jphysiol.2008.158253. Epub 2008 Jul 31. J Physiol. 2008. PMID: 18669532 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources