Sister chromosome cohesion of Escherichia coli - PubMed (original) (raw)
Sister chromosome cohesion of Escherichia coli
Y Sunako et al. Mol Microbiol. 2001 Dec.
Free article
Abstract
We analysed Escherichia coli cells synchronized for initiation of chromosomal DNA replication by fluorescence in situ hybridization (FISH) using fluorescent DNA probes corresponding to various chromosomal regions. Sister copies of regions in an approximately oriC-proximal half of the chromosome are cohesive with each other after replication until the late period of chromosome replication. Sister copies of regions relatively close to the terminus are also separated from each other in the same late period of replication. It is important that sister copies in all the tested regions are thus separated from each other nearly all at once in the late period of chromosome replication. These results are consistent with results obtained by FISH in randomly growing cultures. Cohesion of sister copies in an oriC-close region is observed in a dam null mutant lacking DNA adenine methyltransferase the same as in the parental isogenic dam+ strain, indicating that the cohesion is independent of DNA adenine methyltransferase. This further implies that hemimethylated DNA-binding proteins, such as SeqA, are not involved in the cohesion. On the other hand, the cohesion of sister copies of the oriC-close region was not observed in mukB null mutant cells, suggesting that MukB might be involved in the chromosome cohesion.
Similar articles
- Dynamic events of sister chromosomes in the cell cycle of Escherichia coli.
Adachi S, Fukushima T, Hiraga S. Adachi S, et al. Genes Cells. 2008 Feb;13(2):181-97. doi: 10.1111/j.1365-2443.2007.01157.x. Genes Cells. 2008. PMID: 18233960 - Chromosomal replication incompatibility in Dam methyltransferase deficient Escherichia coli cells.
Løbner-Olesen A, von Freiesleben U. Løbner-Olesen A, et al. EMBO J. 1996 Nov 1;15(21):5999-6008. EMBO J. 1996. PMID: 8918477 Free PMC article. - Behavior of sister copies of mini-F plasmid after synchronized plasmid replication in Escherichia coli cells.
Onogi T, Miki T, Hiraga S. Onogi T, et al. J Bacteriol. 2002 Jun;184(11):3142-5. doi: 10.1128/JB.184.11.3142-3145.2002. J Bacteriol. 2002. PMID: 12003959 Free PMC article. - Partitioning of the Escherichia coli chromosome: superhelicity and condensation.
Nordström K, Dasgupta S. Nordström K, et al. Biochimie. 2001 Jan;83(1):41-8. doi: 10.1016/s0300-9084(00)01204-9. Biochimie. 2001. PMID: 11254973 Review. - Dynamic localization of bacterial and plasmid chromosomes.
Hiraga S. Hiraga S. Annu Rev Genet. 2000;34:21-59. doi: 10.1146/annurev.genet.34.1.21. Annu Rev Genet. 2000. PMID: 11092821 Review.
Cited by
- Chromosome organization shapes replisome dynamics in Caulobacter crescentus.
Zhang C, Joseph AM, Casini L, Collier J, Badrinarayanan A, Manley S. Zhang C, et al. Nat Commun. 2024 Apr 24;15(1):3460. doi: 10.1038/s41467-024-47849-6. Nat Commun. 2024. PMID: 38658616 Free PMC article. - The SMC-like RecN protein is at the crossroads of several genotoxic stress responses in Escherichia coli.
Camus A, Espinosa E, Zapater Baras P, Singh P, Quenech'Du N, Vickridge E, Modesti M, Barre FX, Espéli O. Camus A, et al. Front Microbiol. 2023 Apr 24;14:1146496. doi: 10.3389/fmicb.2023.1146496. eCollection 2023. Front Microbiol. 2023. PMID: 37168111 Free PMC article. - Competitive Coherence Generates Qualia in Bacteria and Other Living Systems.
Norris V. Norris V. Biology (Basel). 2021 Oct 12;10(10):1034. doi: 10.3390/biology10101034. Biology (Basel). 2021. PMID: 34681133 Free PMC article. - Generation of Bacterial Diversity by Segregation of DNA Strands.
Norris V, Ripoll C. Norris V, et al. Front Microbiol. 2021 Mar 22;12:550856. doi: 10.3389/fmicb.2021.550856. eCollection 2021. Front Microbiol. 2021. PMID: 33828535 Free PMC article. - Z-Ring-Associated Proteins Regulate Clustering of the Replication Terminus-Binding Protein ZapT in Caulobacter crescentus.
Ozaki S, Wakasugi Y, Katayama T. Ozaki S, et al. mBio. 2021 Jan 26;12(1):e02196-20. doi: 10.1128/mBio.02196-20. mBio. 2021. PMID: 33500340 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials