Analysis of the Bacillus subtilis spoIIIJ gene and its Paralogue gene, yqjG - PubMed (original) (raw)

FIG.3.

Localization of SpoIIIJ-GFP and YqjG-GFP fusion proteins. (A) Typical phase-contrast (a, c, e, g, i, k, m, and o) and fluorescence (b, d, f, h, j, l, n, and p) micrographs are shown. Strains carrying SpoIIIJ-GFP (a to h, strain JGFP) and YqjG-GFP (i to p, strain QGFP) were observed in the vegetative stage (a, b, i, and j) and at _t_2 (c, d, k, and l), _t_3 (e, f, m, and n), and _t_4 (g, h, o, and p). (B) Western blots of cell extracts were analyzed with an antibody that recognizes GFP. Lanes 1, insoluble fraction; lanes 2, soluble fraction.