A screen for genes expressed in Drosophila imaginal discs - PubMed (original) (raw)
Affiliations
- PMID: 11902680
Free article
A screen for genes expressed in Drosophila imaginal discs
Offer Gerlitz et al. Int J Dev Biol. 2002 Jan.
Free article
Abstract
The development of Drosophila imaginal discs serves as a model system to understand how genes determine the shape and size of an organ. The identification of genes involved in this process is an important step towards this goal. Here we describe a P-element based enhancer trap screen for genes expressed in the larval imaginal discs. Our aim was to establish a large collection of enhancer trap lines each showing expression of Gal4 in imaginal discs. To this end, we improved the well established P-element vector pGawB in order to obtain higher in vivo transposition frequencies. In addition we chose an F1-screening approach using UAS-GFP as a reporter gene. This system permits the efficient screening of larval and pupal stages of living animals and the detection of imaginal gene expression patterns through the transparent cuticle. The procedure has been optimized for high-throughput. 2'000 P-element insertions have been established which exhibit expression in imaginal discs.
Similar articles
- GAL4 enhancer traps expressed in the embryo, larval brain, imaginal discs, and ovary of Drosophila.
Manseau L, Baradaran A, Brower D, Budhu A, Elefant F, Phan H, Philp AV, Yang M, Glover D, Kaiser K, Palter K, Selleck S. Manseau L, et al. Dev Dyn. 1997 Jul;209(3):310-22. doi: 10.1002/(SICI)1097-0177(199707)209:3<310::AID-AJA6>3.0.CO;2-L. Dev Dyn. 1997. PMID: 9215645 - Targeted gene expression by the Gal4-UAS system in zebrafish.
Asakawa K, Kawakami K. Asakawa K, et al. Dev Growth Differ. 2008 Aug;50(6):391-9. doi: 10.1111/j.1440-169X.2008.01044.x. Epub 2008 May 14. Dev Growth Differ. 2008. PMID: 18482403 - Genomic mapping and expression patterns of C380, OK6 and D42 enhancer trap lines in the larval nervous system of Drosophila.
Sanyal S. Sanyal S. Gene Expr Patterns. 2009 Jun;9(5):371-80. doi: 10.1016/j.gep.2009.01.002. Gene Expr Patterns. 2009. PMID: 19602393 - Ectopic gene expression in Drosophila using GAL4 system.
Phelps CB, Brand AH. Phelps CB, et al. Methods. 1998 Apr;14(4):367-79. doi: 10.1006/meth.1998.0592. Methods. 1998. PMID: 9608508 Review. - GAL4 system in Drosophila: a fly geneticist's Swiss army knife.
Duffy JB. Duffy JB. Genesis. 2002 Sep-Oct;34(1-2):1-15. doi: 10.1002/gene.10150. Genesis. 2002. PMID: 12324939 Review. No abstract available.
Cited by
- Enhancer trap infidelity in Drosophila optomotor-blind.
Mayer LR, Diegelmann S, Abassi Y, Eichinger F, Pflugfelder GO. Mayer LR, et al. Fly (Austin). 2013 Apr-Jun;7(2):118-28. doi: 10.4161/fly.23657. Epub 2013 Mar 21. Fly (Austin). 2013. PMID: 23519069 Free PMC article. - microRNA regulation of Wnt signaling pathways in development and disease.
Song JL, Nigam P, Tektas SS, Selva E. Song JL, et al. Cell Signal. 2015 Jul;27(7):1380-91. doi: 10.1016/j.cellsig.2015.03.018. Epub 2015 Apr 2. Cell Signal. 2015. PMID: 25843779 Free PMC article. Review. - Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon.
Geurts AM, Wilber A, Carlson CM, Lobitz PD, Clark KJ, Hackett PB, McIvor RS, Largaespada DA. Geurts AM, et al. BMC Biotechnol. 2006 Jun 26;6:30. doi: 10.1186/1472-6750-6-30. BMC Biotechnol. 2006. PMID: 16800892 Free PMC article. - Reggie-1/flotillin-2 promotes secretion of the long-range signalling forms of Wingless and Hedgehog in Drosophila.
Katanaev VL, Solis GP, Hausmann G, Buestorf S, Katanayeva N, Schrock Y, Stuermer CA, Basler K. Katanaev VL, et al. EMBO J. 2008 Feb 6;27(3):509-21. doi: 10.1038/sj.emboj.7601981. Epub 2008 Jan 24. EMBO J. 2008. PMID: 18219274 Free PMC article. - Establishment of transgenic lines for jumpstarter method using a composite transposon vector in the ladybird beetle, Harmonia axyridis.
Kuwayama H, Gotoh H, Konishi Y, Nishikawa H, Yaginuma T, Niimi T. Kuwayama H, et al. PLoS One. 2014 Jun 24;9(6):e100804. doi: 10.1371/journal.pone.0100804. eCollection 2014. PLoS One. 2014. PMID: 24959904 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases