PilC of pathogenic Neisseria is associated with the bacterial cell surface - PubMed (original) (raw)
PilC of pathogenic Neisseria is associated with the bacterial cell surface
M Rahman et al. Mol Microbiol. 1997 Jul.
Free article
Abstract
Adherence of pathogenic Neisseria to target host cells is mediated by pili. PilC1 and PilC2 are two high-molecular-weight proteins involved in pilus assembly and cellular adherence functions of the pili. Inactivation of pilC1 or pilC2 in N. meningitidis resulted in clones that expressed the same number of pili as the parent, contained no alterations in pilE and showed no detectable differences in PilE glycosylation. However, the PilC2+ pilC1- mutant showed much reduced adherence to target cells, indicating that production of PilC1 is essential for pilus-mediated adherence. To study further the functional differences between the meningococcal pilC genes, we determined the complete nucleotide sequence of pilC1 and pilC2 of N. meningitidis. Alignment of six PilC sequences demonstrated that PilC is composed of both conserved and variable regions. By immunogold labelling of bacterial sections we showed that PilC is present in the membranes of both piliated and non-piliated bacteria. Further, we demonstrated that PilC is associated with the bacterial cell surface.
Similar articles
- Pilus biogenesis and epithelial cell adherence of Neisseria gonorrhoeae pilC double knock-out mutants.
Rudel T, Boxberger HJ, Meyer TF. Rudel T, et al. Mol Microbiol. 1995 Sep;17(6):1057-71. doi: 10.1111/j.1365-2958.1995.mmi_17061057.x. Mol Microbiol. 1995. PMID: 8594326 - The adhesive property of the type IV pilus-associated component PilC1 of pathogenic Neisseria is supported by the conformational structure of the N-terminal part of the molecule.
Morand PC, Tattevin P, Eugene E, Beretti JL, Nassif X. Morand PC, et al. Mol Microbiol. 2001 May;40(4):846-56. doi: 10.1046/j.1365-2958.2001.02452.x. Mol Microbiol. 2001. PMID: 11401692 - Type-4 pili and meningococcal adhesiveness.
Nassif X, Marceau M, Pujol C, Pron B, Beretti JL, Taha MK. Nassif X, et al. Gene. 1997 Jun 11;192(1):149-53. doi: 10.1016/s0378-1119(96)00802-5. Gene. 1997. PMID: 9224885 Review. - Transformation competence and type-4 pilus biogenesis in Neisseria gonorrhoeae--a review.
Fussenegger M, Rudel T, Barten R, Ryll R, Meyer TF. Fussenegger M, et al. Gene. 1997 Jun 11;192(1):125-34. doi: 10.1016/s0378-1119(97)00038-3. Gene. 1997. PMID: 9224882 Review.
Cited by
- Calcium binding properties of the Kingella kingae PilC1 and PilC2 proteins have differential effects on type IV pilus-mediated adherence and twitching motility.
Porsch EA, Johnson MD, Broadnax AD, Garrett CK, Redinbo MR, St Geme JW 3rd. Porsch EA, et al. J Bacteriol. 2013 Feb;195(4):886-95. doi: 10.1128/JB.02186-12. Epub 2012 Dec 14. J Bacteriol. 2013. PMID: 23243304 Free PMC article. - CD46-independent binding of neisserial type IV pili and the major pilus adhesin, PilC, to human epithelial cells.
Kirchner M, Heuer D, Meyer TF. Kirchner M, et al. Infect Immun. 2005 May;73(5):3072-82. doi: 10.1128/IAI.73.5.3072-3082.2005. Infect Immun. 2005. PMID: 15845515 Free PMC article. - Targeting of the pro-apoptotic VDAC-like porin (PorB) of Neisseria gonorrhoeae to mitochondria of infected cells.
Müller A, Günther D, Brinkmann V, Hurwitz R, Meyer TF, Rudel T. Müller A, et al. EMBO J. 2000 Oct 16;19(20):5332-43. doi: 10.1093/emboj/19.20.5332. EMBO J. 2000. PMID: 11032801 Free PMC article. - Identification of regions of the chromosome of Neisseria meningitidis and Neisseria gonorrhoeae which are specific to the pathogenic Neisseria species.
Perrin A, Nassif X, Tinsley C. Perrin A, et al. Infect Immun. 1999 Nov;67(11):6119-29. doi: 10.1128/IAI.67.11.6119-6129.1999. Infect Immun. 1999. PMID: 10531275 Free PMC article. - Complete Genome Sequence of Serogroup B Neisseria meningitidis Strain C311.
Jen FE, Atack JM, Zhang Y, Edwards JL, Jennings MP. Jen FE, et al. Microbiol Resour Announc. 2021 Oct 21;10(42):e0078821. doi: 10.1128/MRA.00788-21. Epub 2021 Oct 21. Microbiol Resour Announc. 2021. PMID: 34672707 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources