Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts - PubMed (original) (raw)
. 2002 Mar 29;295(5564):2452-6.
doi: 10.1126/science.1068798.
Affiliations
- PMID: 11923538
- DOI: 10.1126/science.1068798
Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts
Petr Kalab et al. Science. 2002.
Abstract
The small guanosine triphosphatase Ran is loaded with guanosine triphosphate (GTP) by the chromatin-bound guanine nucleotide exchange factor RCC1 and releases import cargoes in the nucleus during interphase. In mitosis, Ran-GTP promotes spindle assembly around chromosomes by locally discharging cargoes that regulate microtubule dynamics and organization. We used fluorescence resonance energy transfer-based biosensors to visualize gradients of Ran-GTP and liberated cargoes around chromosomes in mitotic Xenopus egg extracts. Both gradients were required to assemble and maintain spindle structure. During interphase, Ran-GTP was highly enriched in the nucleoplasm, and a steep concentration difference between nuclear and cytoplasmic Ran-GTP was established, providing evidence for a Ran-GTP gradient surrounding chromosomes throughout the cell cycle.
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