Gene expression pattern in Caco-2 cells following rotavirus infection - PubMed (original) (raw)

Gene expression pattern in Caco-2 cells following rotavirus infection

Mariela A Cuadras et al. J Virol. 2002 May.

Abstract

Rotaviruses are recognized as the leading cause of severe dehydrating diarrhea in infants and young children worldwide. Preventive and therapeutic strategies are urgently needed to fight this pathogen. In tissue culture and in vivo, rotavirus induces structural and functional alterations in the host cell. In order to better understand the molecular mechanisms involved in the events after rotavirus infection, we identified host cellular genes whose mRNA levels changed after infection. For this analysis, we used microarrays containing more than 38,000 human cDNAs to study the transcriptional response of the human intestinal cell line Caco-2 to rotavirus infection. We found that 508 genes were differentially regulated >2-fold at 16 h after rotavirus infection, and only one gene was similarly regulated at 1 h postinfection. Of these transcriptional changes, 73% corresponded to the upregulation of genes, with the majority of them occurring late, at 12 or more hours postinfection. Some of the regulated genes were classified according to known biological function and included genes encoding integral membrane proteins, interferon-regulated genes, transcriptional and translational regulators, and calcium metabolism-related genes. A new picture of global transcriptional regulation in the infected cell is presented and families of genes which may be involved in viral pathogenesis are discussed.

PubMed Disclaimer

References

    1. Angel, J., M. A. Franco, H. B. Greenberg, and D. Bass. 1999. Lack of a role for type I and type II interferons in the resolution of rotavirus-induced diarrhea and infection in mice J. Interferon Cytokine Res 19:655-659. - PubMed
    1. Ball, J. M., P. Tian, C. Q. Zeng, A. P. Morris, and M. K. Estes. 1996. Age-dependent diarrhea induced by a rotaviral nonstructural glycoprotein Science 272:101-104. - PubMed
    1. Bass, D. M. 1997. Interferon gamma and interleukin 1, but not interferon alpha, inhibit rotavirus entry into human intestinal cell lines. Gastroenterology 113:81-89. - PMC - PubMed
    1. Bishop, R. F., G. P. Davidson, I. H. Holmes, and B. J. Ruck. 1973. Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet ii:1281-1283. - PubMed
    1. Brunet, J. P., J. Cotte-Laffitte, C. Linxe, A. M. Quero, M. Geniteau-Legendre, and A. Servin. 2000. Rotavirus infection induces an increase in intracellular calcium concentration in human intestinal epithelial cells: role in microvillar actin alteration. J. Virol 74:2323-2332. - PMC - PubMed

Publication types

MeSH terms

Substances

Grants and funding

LinkOut - more resources