Activation of the cation channel long transient receptor potential channel 2 (LTRPC2) by hydrogen peroxide. A splice variant reveals a mode of activation independent of ADP-ribose - PubMed (original) (raw)
. 2002 Jun 28;277(26):23150-6.
doi: 10.1074/jbc.M112096200. Epub 2002 Apr 17.
Affiliations
- PMID: 11960981
- DOI: 10.1074/jbc.M112096200
Free article
Activation of the cation channel long transient receptor potential channel 2 (LTRPC2) by hydrogen peroxide. A splice variant reveals a mode of activation independent of ADP-ribose
Edith Wehage et al. J Biol Chem. 2002.
Free article
Abstract
LTRPC2 is a cation channel recently reported to be activated by adenosine diphosphate-ribose (ADP-ribose) and NAD. Since ADP-ribose can be formed from NAD and NAD is elevated during oxidative stress, we studied whole cell currents and increases in the intercellular free calcium concentration ([Ca(2+)](i)) in long transient receptor potential channel 2 (LTRPC2)-transfected HEK 293 cells after stimulation with hydrogen peroxide (H(2)O(2)). Cation currents carried by monovalent cations and Ca(2+) were induced by H(2)O(2) (5 mm in the bath solution) as well as by intracellular ADP-ribose (0.3 mm in the pipette solution) but not by NAD (1 mm). H(2)O(2)-induced currents developed slowly after a characteristic delay of 3-6 min and receded after wash-out of H(2)O(2). [Ca(2+)](i) was rapidly increased by H(2)O(2) in LTRPC2-transfected cells as well as in control cells; however, in LTRPC2-transfected cells, H(2)O(2) evoked a second delayed rise in [Ca(2+)](i). A splice variant of LTRPC2 with a deletion in the C terminus (amino acids 1292-1325) was identified in neutrophil granulocytes. This variant was stimulated by H(2)O(2) as the wild type. However, it did not respond to ADP-ribose. We conclude that activation of LTRPC2 by H(2)O(2) is independent of ADP-ribose and that LTRPC2 may mediate the influx of Na(+) and Ca(2+) during oxidative stress, such as the respiratory burst in granulocytes.
Similar articles
- Expression profile of the transient receptor potential (TRP) family in neutrophil granulocytes: evidence for currents through long TRP channel 2 induced by ADP-ribose and NAD.
Heiner I, Eisfeld J, Halaszovich CR, Wehage E, Jüngling E, Zitt C, Lückhoff A. Heiner I, et al. Biochem J. 2003 May 1;371(Pt 3):1045-53. doi: 10.1042/BJ20021975. Biochem J. 2003. PMID: 12564954 Free PMC article. - ADP-ribose gating of the calcium-permeable LTRPC2 channel revealed by Nudix motif homology.
Perraud AL, Fleig A, Dunn CA, Bagley LA, Launay P, Schmitz C, Stokes AJ, Zhu Q, Bessman MJ, Penner R, Kinet JP, Scharenberg AM. Perraud AL, et al. Nature. 2001 May 31;411(6837):595-9. doi: 10.1038/35079100. Nature. 2001. PMID: 11385575 - Immunocyte Ca2+ influx system mediated by LTRPC2.
Sano Y, Inamura K, Miyake A, Mochizuki S, Yokoi H, Matsushime H, Furuichi K. Sano Y, et al. Science. 2001 Aug 17;293(5533):1327-30. doi: 10.1126/science.1062473. Science. 2001. PMID: 11509734 - TRPM2: a calcium influx pathway regulated by oxidative stress and the novel second messenger ADP-ribose.
Kühn FJ, Heiner I, Lückhoff A. Kühn FJ, et al. Pflugers Arch. 2005 Oct;451(1):212-9. doi: 10.1007/s00424-005-1446-y. Epub 2005 Jun 11. Pflugers Arch. 2005. PMID: 15952035 Review. - Role and regulation of TRP channels in neutrophil granulocytes.
Heiner I, Eisfeld J, Lückhoff A. Heiner I, et al. Cell Calcium. 2003 May-Jun;33(5-6):533-40. doi: 10.1016/s0143-4160(03)00058-7. Cell Calcium. 2003. PMID: 12765698 Review.
Cited by
- Thiol dependent intramolecular locking of Orai1 channels.
Alansary D, Schmidt B, Dörr K, Bogeski I, Rieger H, Kless A, Niemeyer BA. Alansary D, et al. Sci Rep. 2016 Sep 14;6:33347. doi: 10.1038/srep33347. Sci Rep. 2016. PMID: 27624281 Free PMC article. - Redox signal-mediated sensitization of transient receptor potential melastatin 2 (TRPM2) to temperature affects macrophage functions.
Kashio M, Sokabe T, Shintaku K, Uematsu T, Fukuta N, Kobayashi N, Mori Y, Tominaga M. Kashio M, et al. Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6745-50. doi: 10.1073/pnas.1114193109. Epub 2012 Apr 9. Proc Natl Acad Sci U S A. 2012. PMID: 22493272 Free PMC article. - The TRPM2 ion channel regulates metabolic and thermogenic adaptations in adipose tissue of cold-exposed mice.
Benzi A, Heine M, Spinelli S, Salis A, Worthmann A, Diercks B, Astigiano C, Pérez Mato R, Memushaj A, Sturla L, Vellone V, Damonte G, Jaeckstein MY, Koch-Nolte F, Mittrücker HW, Guse AH, De Flora A, Heeren J, Bruzzone S. Benzi A, et al. Front Endocrinol (Lausanne). 2024 Feb 6;14:1251351. doi: 10.3389/fendo.2023.1251351. eCollection 2024. Front Endocrinol (Lausanne). 2024. PMID: 38390373 Free PMC article. - Effects of Hyperthermia on TRPV1 and TRPV4 Channels Expression and Oxidative Markers in Mouse Brain.
Aghazadeh A, Feizi MAH, Fanid LM, Ghanbari M, Roshangar L. Aghazadeh A, et al. Cell Mol Neurobiol. 2021 Oct;41(7):1453-1465. doi: 10.1007/s10571-020-00909-z. Epub 2020 Jul 13. Cell Mol Neurobiol. 2021. PMID: 32661579 - TRPM channels in health and disease.
Chubanov V, Köttgen M, Touyz RM, Gudermann T. Chubanov V, et al. Nat Rev Nephrol. 2024 Mar;20(3):175-187. doi: 10.1038/s41581-023-00777-y. Epub 2023 Oct 18. Nat Rev Nephrol. 2024. PMID: 37853091 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous