The prophage of SP beta c2dcitK1, A defective specialized transducing phage of Bacillus subtilis - PubMed (original) (raw)
The prophage of SP beta c2dcitK1, A defective specialized transducing phage of Bacillus subtilis
R Rosenthal et al. Genetics. 1979 Jul.
Abstract
The defective specialized transducing phage SP beta c2dcitK1 carries two known bacterial genes, kauA and citK, as well as SP beta hage markers including the heat-sensitive repressor allele, c2. Some phage genes (including essential ones) are missing. When SP beta c2dcitK1 transduces SP beta-sensitive cells of Bacillus subtilis, the defective prophage is inserted into sites in the homologous bacterial DNA of the attSP beta-kauA-citK region of the recipient chromosome. During the growth of these transductants, occasional excisions occur that result in the loss of the phage genes and of the heterogenotic state. These excisions increase greatly in frequency during growth at repressor-inactivating temperatures. The kinds of insertions and excisions seen suggest that a Campbell-type (CAMPBELL 1962) circular phage genome may occur transiently. If the transductants are superinfected by SP beta c2 or by the clear-plaque mutant SP beta c1, the resulting double lysogen can be heat induced to release high-frequency-of-transduction (HFT) lysates for kauA and citK.
Similar articles
- Specialized transduction of the ilvD-thyB-ilvA region mediated by Bacillus subtilis bacteriophage SP beta.
Fink PS, Zahler SA. Fink PS, et al. J Bacteriol. 1982 Jun;150(3):1274-9. doi: 10.1128/jb.150.3.1274-1279.1982. J Bacteriol. 1982. PMID: 6804441 Free PMC article. - Insertion of bacteriophage SP beta into the citF gene of Bacillus subtilis and specialized transduction of the ilvBC-leu genes.
Mackey CJ, Zahler SA. Mackey CJ, et al. J Bacteriol. 1982 Sep;151(3):1222-9. doi: 10.1128/jb.151.3.1222-1229.1982. J Bacteriol. 1982. PMID: 6809729 Free PMC article. - Cloning of the Bacillus subtilis lys and spoIIIB genes in phage phi 105.
Jenkinson HF, Mandelstam J. Jenkinson HF, et al. J Gen Microbiol. 1983 Jul;129(7):2229-40. doi: 10.1099/00221287-129-7-2229. J Gen Microbiol. 1983. PMID: 6313855 - Bacteriophages of Bacillus subtilis.
Hemphill HE, Whiteley HR. Hemphill HE, et al. Bacteriol Rev. 1975 Sep;39(3):257-315. doi: 10.1128/br.39.3.257-315.1975. Bacteriol Rev. 1975. PMID: 809034 Free PMC article. Review. No abstract available. - Coevolution of bacteria and their viruses.
Golais F, Hollý J, Vítkovská J. Golais F, et al. Folia Microbiol (Praha). 2013 May;58(3):177-86. doi: 10.1007/s12223-012-0195-5. Epub 2012 Sep 21. Folia Microbiol (Praha). 2013. PMID: 22993102 Review.
Cited by
- Phages overcome bacterial immunity via diverse anti-defence proteins.
Yirmiya E, Leavitt A, Lu A, Ragucci AE, Avraham C, Osterman I, Garb J, Antine SP, Mooney SE, Hobbs SJ, Kranzusch PJ, Amitai G, Sorek R. Yirmiya E, et al. Nature. 2024 Jan;625(7994):352-359. doi: 10.1038/s41586-023-06869-w. Epub 2023 Nov 22. Nature. 2024. PMID: 37992756 - Characterization of a FourU RNA Thermometer in the 5' Untranslated Region of Autolysin Gene blyA in the Bacillus subtilis 168 Prophage SPβ.
Tong AY, Caudill EE, Jones AR, F M Passalacqua L, Abdelsayed MM. Tong AY, et al. Biochemistry. 2023 Oct 17;62(20):2902-2907. doi: 10.1021/acs.biochem.3c00368. Epub 2023 Sep 12. Biochemistry. 2023. PMID: 37699513 Free PMC article. - Structural and functional characterization of MrpR, the master repressor of the Bacillus subtilis prophage SPβ.
Kohm K, Jalomo-Khayrova E, Krüger A, Basu S, Steinchen W, Bange G, Frunzke J, Hertel R, Commichau FM, Czech L. Kohm K, et al. Nucleic Acids Res. 2023 Sep 22;51(17):9452-9474. doi: 10.1093/nar/gkad675. Nucleic Acids Res. 2023. PMID: 37602373 Free PMC article. - The life cycle of SPβ and related phages.
Kohm K, Hertel R. Kohm K, et al. Arch Virol. 2021 Aug;166(8):2119-2130. doi: 10.1007/s00705-021-05116-9. Epub 2021 Jun 7. Arch Virol. 2021. PMID: 34100162 Free PMC article. Review. - Complete Genome Sequence of Bacillus subtilis Strain CU1050, Which Is Sensitive to Phage SPβ.
Johnson CM, Grossman AD. Johnson CM, et al. Genome Announc. 2016 Apr 7;4(2):e00262-16. doi: 10.1128/genomeA.00262-16. Genome Announc. 2016. PMID: 27056236 Free PMC article.
References
- Virology. 1957 Feb;3(1):42-61 - PubMed
- Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 - PubMed
- J Bacteriol. 1973 Nov;116(2):719-26 - PubMed
- J Bacteriol. 1976 Jul;127(1):667-70 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous