Salmonella enterica serotype Typhimurium DT 104 antibiotic resistance genomic island I in serotype paratyphi B - PubMed (original) (raw)

Salmonella enterica serotype Typhimurium DT 104 antibiotic resistance genomic island I in serotype paratyphi B

Danièle Meunier et al. Emerg Infect Dis. 2002 Apr.

Abstract

We have identified Salmonella genomic island I (SGI1) in an isolate of Salmonella enterica serotype Paratyphi B. This antibiotic-resistance gene cluster, which confers multidrug resistance, has been previously identified in S. enterica serotype Typhimurium phage types DT 104 and DT 120 and in S. enterica serotype Agona.

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Figures

Figure 1

Figure 1

Genetic organization of the antibiotic-resistance gene cluster of SGI1 of Salmonella enterica serotype Typhimurium DT 104. DR-L and DR-R are the left and right direct repeats, respectively, bracketing SGI1. Polymerase chain reactions (PCRs) used to assess the genetic organization of the antibiotic-resistance genes (PCRs floR, A, B, C, D, and E) and the SGI1 junctions to the chromosome (PCRs LJ and RJ for left and right junctions, respectively) are indicated. Abbreviations for restriction sites: X, _Xba_I; H, _Hin_dIII; Xh, _Xho_I.

Figure 2

Figure 2

A. Southern blot hybridization with the _Xba_I probe (Figure 1) containing all antibiotic-resistance genes of _Hin_dIII- and _Xho_I-digested genomic DNAs of Salmonella enterica serotype Typhimurium DT 104 strain BN9181 (lanes 2), serotype Agona strain 959SA97 (lanes 3), and serotype Paratyphi B strain (lanes 4). Lane 1: DNA ladder. B. Southern blot hybridization with the p1-9 probe of _Xba_I-digested genomic DNAs of S. enterica serotype Typhimurium DT 104 strain BN9181 (lanes 2), serotype Agona strain 959SA97 (lanes 3), and serotype Paratyphi B strain (lanes 4). Lane 1: DNA ladder.

Figure 3

Figure 3

Macrorestriction analysis by pulsed-field gel electrophoresis of genomic DNAs cut by _Xba_I of S. enterica serotype Typhimurium DT 104 strain BN9181 (lane 2), serotype Agona strain 959SA97 (lane 3), and the serotype Paratyphi B strain (lane 4). Lane 1: DNA ladder.

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