Acetylation of beta-catenin by CREB-binding protein (CBP) - PubMed (original) (raw)
. 2002 Jul 12;277(28):25562-7.
doi: 10.1074/jbc.M201196200. Epub 2002 Apr 24.
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- PMID: 11973335
- DOI: 10.1074/jbc.M201196200
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Acetylation of beta-catenin by CREB-binding protein (CBP)
Daniel Wolf et al. J Biol Chem. 2002.
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Abstract
Acetylation controls the activity of numerous proteins involved in regulating gene transcription as well as many other cellular processes. In this report we show that the CREB-binding protein (CBP) acetyltransferase acetylates beta-catenin protein in vivo. beta-Catenin is a central component of the Wnt signaling pathway, which is of key importance in development as well as being heavily implicated in a variety of human cancers. We show that the CBP-mediated acetylation of beta-catenin occurs at a single site, lysine 49. Importantly, this lysine is frequently found mutated in cancer and is in a region of importance to the regulation of beta-catenin. We show that mutation of this site leads specifically to an increase in the ability of beta-catenin to activate the c-myc gene but not other beta-catenin-regulated genes. This suggests that acetylation of beta-catenin is involved in regulating Wnt signaling in a promoter-specific fashion.
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