GATEWAY vectors for Agrobacterium-mediated plant transformation - PubMed (original) (raw)
GATEWAY vectors for Agrobacterium-mediated plant transformation
Mansour Karimi et al. Trends Plant Sci. 2002 May.
Abstract
Agrobacterium tumefaciens is the preferred method for transformation of a wide range of plant species. Commonly, the genes to be transferred are cloned between the left and right T-DNA borders of so-called binary T-DNA vectors that can replicate both in E. coli and Agrobacterium. Because these vectors are generally large, cloning can be time-consuming and laborious. Recently, the GATEWAY conversion technology has provided a fast and reliable alternative to the cloning of sequences into large acceptor plasmids.
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