CXCR5-transduced bone marrow-derived dendritic cells traffic to B cell zones of lymph nodes and modify antigen-specific immune responses - PubMed (original) (raw)

CXCR5-transduced bone marrow-derived dendritic cells traffic to B cell zones of lymph nodes and modify antigen-specific immune responses

Meng-Tse Wu et al. J Immunol. 2002.

Abstract

Skin-derived migratory dendritic cells (DC), in contrast to bone marrow-derived DC (BMDC), express CXCR5, respond to the chemokine CXC ligand 13 (CXCL13) in vitro, and are capable of migrating to B cell zones (BCZ) in lymph nodes (LN) in vivo. Herein, we analyzed the surface phenotype of skin-derived migratory DC and found that 15-35% of MHC class II(high) cells showed high levels of expression of CXCR5 but expressed low levels of DEC205, a suggested characteristic of dermal-type DC in mice. To study the effects of CXCR5 on the trafficking dynamics of DC, we stably expressed CXCR5 in BMDC by retroviral gene transduction. CXCR5 was detected by flow cytometry on transduced cells, which responded to CXCL13 in vitro in chemotaxis assays (3-fold over nontransduced BMDC, p < 0.01). When injected into the footpads of mice, approximately 40% of injected CXCR5-BMDC were observed in BCZ of draining LN. Mice were vaccinated with CXCR5- and vector-BMDC that were pulsed with keyhole limpet hemocyanin (KLH) to induce Ag-specific cellular and humoral immune responses. Mice injected with CXCR5-BMDC (vs vector-BMDC) demonstrated marginally less footpad swelling in response to intradermal injection of KLH. Interestingly, significantly higher levels of KLH-specific IgG (p < 0.05) and IgM (p < 0.01) were found in the serum of mice injected with CXCR5-BMDC compared with mice immunized with vector-transduced BMDC. Thus, CXCR5 is predominantly expressed by dermal-type DC. Moreover, CXCR5 directs BMDC to BCZ of LN in vivo and modifies Ag-specific immune responses induced by BMDC vaccination.

PubMed Disclaimer

Cited by

Activation of IKKalpha target genes depends on recognition of specific kappaB binding sites by RelB:p52 dimers.
Bonizzi G, Bebien M, Otero DC, Johnson-Vroom KE, Cao Y, Vu D, Jegga AG, Aronow BJ, Ghosh G, Rickert RC, Karin M. Bonizzi G, et al. EMBO J. 2004 Oct 27;23(21):4202-10. doi: 10.1038/sj.emboj.7600391. Epub 2004 Oct 7. EMBO J. 2004. PMID: 15470505 Free PMC article.
Role of G protein-coupled receptors in control of dendritic cell migration.
Liu Y, Shi G. Liu Y, et al. Biomed Res Int. 2014;2014:738253. doi: 10.1155/2014/738253. Epub 2014 Mar 10. Biomed Res Int. 2014. PMID: 24734242 Free PMC article. Review.
Fibroblast growth factor receptor 4 polymorphisms and the prognosis of non-Hodgkin lymphoma.
Cha Z, Zang Y, Guo H, Gu H, Tu X, Song H, Qian B. Cha Z, et al. Mol Biol Rep. 2014 Feb;41(2):1165-70. doi: 10.1007/s11033-013-2963-y. Epub 2014 Jan 1. Mol Biol Rep. 2014. PMID: 24381107 Retracted.
Compartmentalization of dendritic cell and T-cell interactions in the lymph node: Anatomy of T-cell fate decisions.
León B, Lund FE. León B, et al. Immunol Rev. 2019 May;289(1):84-100. doi: 10.1111/imr.12758. Immunol Rev. 2019. PMID: 30977197 Free PMC article. Review.
Fibroblast growth factor receptor 4 polymorphisms and coronary artery disease: a case control study.
Zhu Q, Liu T. Zhu Q, et al. Mol Biol Rep. 2012 Sep;39(9):8679-85. doi: 10.1007/s11033-012-1723-8. Epub 2012 Jun 14. Mol Biol Rep. 2012. PMID: 22696188 Retracted.

CXCR5-transduced bone marrow-derived dendritic cells traffic to B cell zones of lymph nodes and modify antigen-specific immune responses - PubMed (original) (raw)