The transcriptional regulator RfaH stimulates RNA chain synthesis after recruitment to elongation complexes by the exposed nontemplate DNA strand - PubMed (original) (raw)

The transcriptional regulator RfaH stimulates RNA chain synthesis after recruitment to elongation complexes by the exposed nontemplate DNA strand

Irina Artsimovitch et al. Cell. 2002.

Free article

Erratum in

Cell 2002 Sep 20;110(6):801

Abstract

The transcriptional regulatory protein RfaH controls expression of several operons that encode extracytoplasmic components in bacteria. Regulation by RfaH occurs during transcript elongation and depends on a 5'-proximal, transcribed nucleic acid sequence called ops that induces transcriptional pausing in vitro and in vivo. We report that RfaH recognizes RNA polymerase transcribing RfaH-regulated operons by interacting with the ops sequence in the exposed nontemplate DNA strand of ops-paused transcription complexes. Although RfaH delays escape from the ops pause, once escape occurs, RfaH enhances elongation by suppressing pausing and rho-dependent termination without apparent involvement of other accessory proteins. This activity predicts a cumulative antitermination model for RfaH's regulation of ops-containing operons in vivo.

PubMed Disclaimer

Cited by

Regulation of Transcript Elongation.
Belogurov GA, Artsimovitch I. Belogurov GA, et al. Annu Rev Microbiol. 2015;69:49-69. doi: 10.1146/annurev-micro-091014-104047. Epub 2015 Jun 24. Annu Rev Microbiol. 2015. PMID: 26132790 Free PMC article. Review.
Comparison of Pause Predictions of Two Sequence-Dependent Transcription Models.
Bai L, Wang MD. Bai L, et al. J Stat Mech. 2010 Dec;2010:P12007. doi: 10.1088/1742-5468/2010/12/P12007. J Stat Mech. 2010. PMID: 22446379 Free PMC article.
The transcriptional antiterminator RfaH represses biofilm formation in Escherichia coli.
Beloin C, Michaelis K, Lindner K, Landini P, Hacker J, Ghigo JM, Dobrindt U. Beloin C, et al. J Bacteriol. 2006 Feb;188(4):1316-31. doi: 10.1128/JB.188.4.1316-1331.2006. J Bacteriol. 2006. PMID: 16452414 Free PMC article.
Functional regions of the N-terminal domain of the antiterminator RfaH.
Belogurov GA, Sevostyanova A, Svetlov V, Artsimovitch I. Belogurov GA, et al. Mol Microbiol. 2010 Apr;76(2):286-301. doi: 10.1111/j.1365-2958.2010.07056.x. Epub 2010 Feb 1. Mol Microbiol. 2010. PMID: 20132437 Free PMC article.
Dynamic competition between a ligand and transcription factor NusA governs riboswitch-mediated transcription regulation.
Chauvier A, Ajmera P, Yadav R, Walter NG. Chauvier A, et al. Proc Natl Acad Sci U S A. 2021 Nov 23;118(47):e2109026118. doi: 10.1073/pnas.2109026118. Proc Natl Acad Sci U S A. 2021. PMID: 34782462 Free PMC article.

The transcriptional regulator RfaH stimulates RNA chain synthesis after recruitment to elongation complexes by the exposed nontemplate DNA strand - PubMed (original) (raw)