C. elegans PAT-4/ILK functions as an adaptor protein within integrin adhesion complexes - PubMed (original) (raw)
C. elegans PAT-4/ILK functions as an adaptor protein within integrin adhesion complexes
A Craig Mackinnon et al. Curr Biol. 2002.
Free article
Abstract
Background: Mammalian integrin-linked kinase (ILK) was identified in a yeast two-hybrid screen for proteins binding the integrin beta(1) subunit cytoplasmic domain. ILK has been implicated in integrin-mediated signaling and is also an adaptor within integrin-associated cytoskeletal complexes.
Results: We identified the C. elegans pat-4 gene in previous genetic screens for mutants unable to assemble integrin-mediated muscle cell attachments. Here, we report that pat-4 encodes the sole C. elegans homolog of ILK. In pat-4 null mutants, embryonic muscle cells form integrin foci, but the subsequent recruitment of vinculin and UNC-89 as well as actin and myosin filaments to these in vivo focal adhesion analogs is blocked. Conversely, PAT-4/ILK requires the ECM component UNC-52/perlecan, the transmembrane protein integrin, and the novel cytoplasmic attachment protein UNC-112 to be properly recruited to nascent attachments. Transgenically expressed "kinase-dead" ILK fully rescues pat-4 loss-of-function mutants. We also identify UNC-112 as a new binding partner for ILK.
Conclusions: Our data strengthens the emerging view that ILK functions primarily as an adaptor protein within integrin adhesion complexes and identifies UNC-112 as a new ILK binding partner.
Comment in
- Integrin adhesion: when is a kinase a kinase?
Zervas CG, Brown NH. Zervas CG, et al. Curr Biol. 2002 May 14;12(10):R350-1. doi: 10.1016/s0960-9822(02)00856-4. Curr Biol. 2002. PMID: 12015134
Similar articles
- UNC-97/PINCH is involved in the assembly of integrin cell adhesion complexes in Caenorhabditis elegans body wall muscle.
Norman KR, Cordes S, Qadota H, Rahmani P, Moerman DG. Norman KR, et al. Dev Biol. 2007 Sep 1;309(1):45-55. doi: 10.1016/j.ydbio.2007.06.014. Epub 2007 Jun 22. Dev Biol. 2007. PMID: 17662976 - The UNC-112 gene in Caenorhabditis elegans encodes a novel component of cell-matrix adhesion structures required for integrin localization in the muscle cell membrane.
Rogalski TM, Mullen GP, Gilbert MM, Williams BD, Moerman DG. Rogalski TM, et al. J Cell Biol. 2000 Jul 10;150(1):253-64. doi: 10.1083/jcb.150.1.253. J Cell Biol. 2000. PMID: 10893272 Free PMC article. - C. elegans PAT-6/actopaxin plays a critical role in the assembly of integrin adhesion complexes in vivo.
Lin X, Qadota H, Moerman DG, Williams BD. Lin X, et al. Curr Biol. 2003 May 27;13(11):922-32. doi: 10.1016/s0960-9822(03)00372-5. Curr Biol. 2003. PMID: 12781130 - How ILK and kindlins cooperate to orchestrate integrin signaling.
Böttcher RT, Lange A, Fässler R. Böttcher RT, et al. Curr Opin Cell Biol. 2009 Oct;21(5):670-5. doi: 10.1016/j.ceb.2009.05.008. Epub 2009 Jun 25. Curr Opin Cell Biol. 2009. PMID: 19560331 Review. - UNC-52/perlecan isoform diversity and function in Caenorhabditis elegans.
Rogalski TM, Mullen GP, Bush JA, Gilchrist EJ, Moerman DG. Rogalski TM, et al. Biochem Soc Trans. 2001 May;29(Pt 2):171-6. doi: 10.1042/0300-5127:0290171. Biochem Soc Trans. 2001. PMID: 11356148 Review.
Cited by
- Requirement for PINCH in skeletal myoblast differentiation.
Liao H, Wang F, Lu K, Ma X, Yan J, Luo L, Sun Y, Liang X. Liao H, et al. Cell Tissue Res. 2023 Jan;391(1):205-215. doi: 10.1007/s00441-022-03701-1. Epub 2022 Nov 17. Cell Tissue Res. 2023. PMID: 36385586 Free PMC article. - Involvement of the extracellular matrix and integrin signalling proteins in skeletal muscle glucose uptake.
Draicchio F, Behrends V, Tillin NA, Hurren NM, Sylow L, Mackenzie R. Draicchio F, et al. J Physiol. 2022 Oct;600(20):4393-4408. doi: 10.1113/JP283039. Epub 2022 Sep 23. J Physiol. 2022. PMID: 36054466 Free PMC article. Review. - Genetic analysis suggests a surface of PAT-4 (ILK) that interacts with UNC-112 (kindlin).
Qadota H, McPherson A, Corbitt R, Dackowski EK, Matsunaga Y, Oberhauser AF, Benian GM. Qadota H, et al. G3 (Bethesda). 2022 Jul 6;12(7):jkac117. doi: 10.1093/g3journal/jkac117. G3 (Bethesda). 2022. PMID: 35536217 Free PMC article. - Integrin-linked kinase (ILK): the known vs. the unknown and perspectives.
Górska A, Mazur AJ. Górska A, et al. Cell Mol Life Sci. 2022 Jan 28;79(2):100. doi: 10.1007/s00018-021-04104-1. Cell Mol Life Sci. 2022. PMID: 35089438 Free PMC article. Review. - Understanding the molecular basis of cardiomyopathy.
Bang ML, Bogomolovas J, Chen J. Bang ML, et al. Am J Physiol Heart Circ Physiol. 2022 Feb 1;322(2):H181-H233. doi: 10.1152/ajpheart.00562.2021. Epub 2021 Nov 19. Am J Physiol Heart Circ Physiol. 2022. PMID: 34797172 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases