Fragmentation and dispersal of the pericentriolar Golgi complex is required for entry into mitosis in mammalian cells - PubMed (original) (raw)

Comparative Study

. 2002 May 3;109(3):359-69.

doi: 10.1016/s0092-8674(02)00720-1.

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• PMID: 12015985
• DOI: 10.1016/s0092-8674(02)00720-1

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Comparative Study

Fragmentation and dispersal of the pericentriolar Golgi complex is required for entry into mitosis in mammalian cells

Christine Sütterlin et al. Cell. 2002.

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Abstract

The pericentriolar Golgi stacks are fragmented and found dispersed in mitotic mammalian cells. Addition of an antibody to the Golgi-associated protein GRASP65 inhibited Golgi fragmentation by mitotic cytosol in permeabilized cells. Microinjecting this antibody or the C-terminal fragment of GRASP65, which contains the antibody binding site, into normal rat kidney cells prevented entry into mitosis. Under these conditions the cells had completed S phase but were not in the prophase stage of mitosis. Fragmentation of the Golgi apparatus by nocodazole or Brefeldin A treatment prior to or post microinjection of the anti-GRASP65 antibody alleviated the block in mitotic entry. Based on our findings, we suggest that the pericentriolar Golgi organization is a sensor for controlling entry into mitosis in mammalian cells.

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