Chromosome-encoded class D beta-lactamase OXA-23 in Proteus mirabilis - PubMed (original) (raw)

Chromosome-encoded class D beta-lactamase OXA-23 in Proteus mirabilis

R Bonnet et al. Antimicrob Agents Chemother. 2002 Jun.

Abstract

Ten nonrepetitive Proteus mirabilis isolates, which were collected over 4 years (1996 to 1999) at the teaching hospital of Clermont-Ferrand, France, produced class D carbapenemase OXA-23. MICs of imipenem were 0.25 to 0.5 microg/ml for these clinical isolates. Molecular typing revealed that the 10 P. mirabilis isolates originated from the same clonal strain. Hybridization of I-CeuI-generated chromosome fragments with a bla(OXA-23) probe showed that the gene was chromosome encoded in the P. mirabilis strain.

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Figures

FIG. 1.

Molecular typing of P. mirabilis isolates. (A) PFGE of _Not_I macrorestriction fragments. (B) Ribotyping. Lanes: 1 to 3, non-OXA-23-producing P. mirabilis strains; 4 to 13, OXA-23-producing P. mirabilis isolates.

FIG. 2.

Localization of _bla_OXA-23 in I-Ceu I-generated chromosome fragments of P. mirabilis CFO239 separated by PFGE. (A) Chromosome restriction patterns. (B) Hybridization of restricted patterns with a probe specific to 23S rRNA genes. (C) Hybridization of restricted patterns with a probe specific to the _bla_OXA-23 gene. Lanes: 1, clinical P. mirabilis without the _bla_OXA-23 gene; 2, OXA-23-producing P. mirabilis CFO239.

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