Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy - PubMed (original) (raw)

. 2002 Jun 7;296(5574):1876-80.

doi: 10.1126/science.1070945.

Affiliations

• PMID: 12052962
• DOI: 10.1126/science.1070945

Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy

Philippe Bousso et al. Science. 2002.

Abstract

Thymocytes are selected to mature according to their ability to interact with self major histocompatibility complex (MHC)-peptide complexes displayed on the thymic stroma. Using two-photon microscopy, we performed real-time analysis of the cellular contacts made by developing thymocytes undergoing positive selection in a three-dimensional thymic organ culture. A large fraction of thymocytes within these cultures were highly motile. MHC recognition was found to increase the duration of thymocyte-stromal cell interactions and occurred as both long-lived cellular associations displaying stable cell-cell contacts and as shorter, highly dynamic contacts. Our results identify the diversity and dynamics of thymocyte interactions during positive selection.

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Comment in

• Immunology. T cell activation in six dimensions.
  von Andrian UH. von Andrian UH. Science. 2002 Jun 7;296(5574):1815-7. doi: 10.1126/science.296.5574.1815. Science. 2002. PMID: 12052942 No abstract available.

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