Disassembly of transcriptional regulatory complexes by molecular chaperones - PubMed (original) (raw)
. 2002 Jun 21;296(5576):2232-5.
doi: 10.1126/science.1073051.
Affiliations
- PMID: 12077419
- DOI: 10.1126/science.1073051
Disassembly of transcriptional regulatory complexes by molecular chaperones
Brian C Freeman et al. Science. 2002.
Abstract
Many biological processes are initiated by cooperative assembly of large multicomponent complexes; however, mechanisms for modulating or terminating the actions of these complexes are not well understood. For example, hormone-bound intracellular receptors (IRs) nucleate formation of transcriptional regulatory complexes whose actions cease promptly upon hormone withdrawal. Here, we show that the p23 molecular chaperone localizes in vivo to genomic response elements in a hormone-dependent manner, disrupting receptor-mediated transcriptional activation in vivo and in vitro; Hsp90 weakly displayed similar activities. Indeed, p23 and Hsp90 also disrupted the activities of some non-IR-containing transcriptional regulatory complexes. We suggest that molecular chaperones promote disassembly of transcriptional regulatory complexes, thus enabling regulatory machineries to detect and respond to signaling changes.
Comment in
- Gene transcription. Demolition crew gets a hand from chaperones.
Marx J. Marx J. Science. 2002 Jun 21;296(5576):2125. doi: 10.1126/science.296.5576.2125. Science. 2002. PMID: 12077382 No abstract available.
Similar articles
- Gene transcription. Demolition crew gets a hand from chaperones.
Marx J. Marx J. Science. 2002 Jun 21;296(5576):2125. doi: 10.1126/science.296.5576.2125. Science. 2002. PMID: 12077382 No abstract available. - The p23 molecular chaperones act at a late step in intracellular receptor action to differentially affect ligand efficacies.
Freeman BC, Felts SJ, Toft DO, Yamamoto KR. Freeman BC, et al. Genes Dev. 2000 Feb 15;14(4):422-34. Genes Dev. 2000. PMID: 10691735 Free PMC article. - Nuclear receptors modulate the interaction of Sp1 and GC-rich DNA via ternary complex formation.
Husmann M, Dragneva Y, Romahn E, Jehnichen P. Husmann M, et al. Biochem J. 2000 Dec 15;352 Pt 3(Pt 3):763-72. Biochem J. 2000. PMID: 11104684 Free PMC article. - Regulation of signaling protein function and trafficking by the hsp90/hsp70-based chaperone machinery.
Pratt WB, Toft DO. Pratt WB, et al. Exp Biol Med (Maywood). 2003 Feb;228(2):111-33. doi: 10.1177/153537020322800201. Exp Biol Med (Maywood). 2003. PMID: 12563018 Review. - Steroid receptor interactions with heat shock protein and immunophilin chaperones.
Pratt WB, Toft DO. Pratt WB, et al. Endocr Rev. 1997 Jun;18(3):306-60. doi: 10.1210/edrv.18.3.0303. Endocr Rev. 1997. PMID: 9183567 Review.
Cited by
- Complex dynamics of transcription regulation.
Stavreva DA, Varticovski L, Hager GL. Stavreva DA, et al. Biochim Biophys Acta. 2012 Jul;1819(7):657-66. doi: 10.1016/j.bbagrm.2012.03.004. Epub 2012 Mar 28. Biochim Biophys Acta. 2012. PMID: 22484099 Free PMC article. Review. - Heterozygous yeast deletion collection screens reveal essential targets of Hsp90.
Franzosa EA, Albanèse V, Frydman J, Xia Y, McClellan AJ. Franzosa EA, et al. PLoS One. 2011;6(11):e28211. doi: 10.1371/journal.pone.0028211. Epub 2011 Nov 30. PLoS One. 2011. PMID: 22140548 Free PMC article. - The Hsp90 cochaperone p23 is essential for perinatal survival.
Grad I, McKee TA, Ludwig SM, Hoyle GW, Ruiz P, Wurst W, Floss T, Miller CA 3rd, Picard D. Grad I, et al. Mol Cell Biol. 2006 Dec;26(23):8976-83. doi: 10.1128/MCB.00734-06. Epub 2006 Sep 25. Mol Cell Biol. 2006. PMID: 17000766 Free PMC article. - Constitutive expression of ZmsHSP in Arabidopsis enhances their cytokinin sensitivity.
Cao Z, Jia Z, Liu Y, Wang M, Zhao J, Zheng J, Wang G. Cao Z, et al. Mol Biol Rep. 2010 Feb;37(2):1089-97. doi: 10.1007/s11033-009-9848-0. Mol Biol Rep. 2010. PMID: 19821154 - Hsp90 modulates CAG repeat instability in human cells.
Mittelman D, Sykoudis K, Hersh M, Lin Y, Wilson JH. Mittelman D, et al. Cell Stress Chaperones. 2010 Sep;15(5):753-9. doi: 10.1007/s12192-010-0191-0. Epub 2010 Apr 8. Cell Stress Chaperones. 2010. PMID: 20373063 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases