Cytokines, chemokines, and cytokine receptors in human microglia - PubMed (original) (raw)
. 2002 Jul 1;69(1):94-103.
doi: 10.1002/jnr.10253.
Affiliations
- PMID: 12111820
- DOI: 10.1002/jnr.10253
Cytokines, chemokines, and cytokine receptors in human microglia
Yong B Lee et al. J Neurosci Res. 2002.
Abstract
Enriched populations of human microglial cells were isolated from mixed cell cultures prepared from embryonic human telencephalon tissues. Human microglial cells exhibited cell type-specific antigens for macrophage-microglia lineage cells including CD11b (Mac-1), CD68, B7-2 (CD86), HLA-ABC, HLA-DR and ricinus communis aggulutinin lectin-1 (RCA-1), and actively phagocytosed latex beads. Gene expression and protein production of cytokines, chemokines and cytokine/chemokine receptors were investigated in the purified populations of human microglia. Normal unstimulated human microglia expressed constitutively mRNA transcripts for interleukin- 1beta (IL-1beta) -6, -8, -10, -12, -15, tumor necrosis factor-alpha (TNF-alpha), macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, and monocyte chemoattractant protein-1 (MCP-1), while treatment with lipopolysaccharide (LPS) or amyloid beta peptides (Abeta) led to increased expression of mRNA levels of IL-8, IL-10, IL-12, TNF-alpha, MIP-1alpha, MIP-1beta, and MCP-1. Human microglia, in addition, expressed mRNA transcripts for IL-1RI, IL-1RII, IL-5R, IL-6R, IL-8R, IL-9R, IL-10R, IL-12R, IL-13R, and IL-15R. Enzyme-linked immunosorbent assays (ELISA) showed increased protein levels in culture media of IL-1beta, IL-8, TNF-alpha, and MIP-1alpha in human microglia following treatment with LPS or Abeta. Increased TNF-alpha release from human microglia following LPS treatment was completely inhibited with IL-10 pretreatment, but not with IL-6, IL-9, IL-12, IL-13, or transforming growth factor-beta (TGF-beta). Present results should help in understanding the basic microglial biology, but also the pathophysiology of activated microglia in neurological diseases such as Alzheimer disease, Parkinson disease, Huntington disease, amyotrophic lateral sclerosis, stroke, and neurotrauma.
Copyright 2002 Wiley-Liss, Inc.
Similar articles
- Generation and characterization of immortalized human microglial cell lines: expression of cytokines and chemokines.
Nagai A, Nakagawa E, Hatori K, Choi HB, McLarnon JG, Lee MA, Kim SU. Nagai A, et al. Neurobiol Dis. 2001 Dec;8(6):1057-68. doi: 10.1006/nbdi.2001.0437. Neurobiol Dis. 2001. PMID: 11741401 - Identification of sequential events and factors associated with microglial activation, migration, and cytotoxicity in retinal degeneration in rd mice.
Zeng HY, Zhu XA, Zhang C, Yang LP, Wu LM, Tso MO. Zeng HY, et al. Invest Ophthalmol Vis Sci. 2005 Aug;46(8):2992-9. doi: 10.1167/iovs.05-0118. Invest Ophthalmol Vis Sci. 2005. PMID: 16043876 - Proinflammatory cytokines and chemokine production and expression by human osteoblasts isolated from patients with rheumatoid arthritis and osteoarthritis.
Lisignoli G, Toneguzzi S, Pozzi C, Piacentini A, Riccio M, Ferruzzi A, Gualtieri G, Facchini A. Lisignoli G, et al. J Rheumatol. 1999 Apr;26(4):791-9. J Rheumatol. 1999. PMID: 10229398 - Immortalized human microglial cell line: phenotypic expression.
Nagai A, Mishima S, Ishida Y, Ishikura H, Harada T, Kobayashi S, Kim SU. Nagai A, et al. J Neurosci Res. 2005 Aug 1;81(3):342-8. doi: 10.1002/jnr.20478. J Neurosci Res. 2005. PMID: 15957187 Review. - Colonisation of the developing human brain and spinal cord by microglia: a review.
Rezaie P, Male D. Rezaie P, et al. Microsc Res Tech. 1999 Jun 15;45(6):359-82. doi: 10.1002/(SICI)1097-0029(19990615)45:6<359::AID-JEMT4>3.0.CO;2-D. Microsc Res Tech. 1999. PMID: 10402264 Review.
Cited by
- Immunomodulatory Effect of Microglia-Released Cytokines in Gliomas.
Lanza M, Casili G, Campolo M, Paterniti I, Colarossi C, Mare M, Giuffrida R, Caffo M, Esposito E, Cuzzocrea S. Lanza M, et al. Brain Sci. 2021 Apr 7;11(4):466. doi: 10.3390/brainsci11040466. Brain Sci. 2021. PMID: 33917013 Free PMC article. Review. - Sympathetic activation: a potential link between comorbidities and COVID-19.
Porzionato A, Emmi A, Barbon S, Boscolo-Berto R, Stecco C, Stocco E, Macchi V, De Caro R. Porzionato A, et al. FEBS J. 2020 Sep;287(17):3681-3688. doi: 10.1111/febs.15481. Epub 2020 Aug 1. FEBS J. 2020. PMID: 32779891 Free PMC article. Review. - IL-9: basic biology, signaling pathways in CD4+ T cells and implications for autoimmunity.
Li H, Rostami A. Li H, et al. J Neuroimmune Pharmacol. 2010 Jun;5(2):198-209. doi: 10.1007/s11481-009-9186-y. Epub 2009 Dec 18. J Neuroimmune Pharmacol. 2010. PMID: 20020328 Review. - Neuroinflammatory processes in Alzheimer's disease.
Heneka MT, O'Banion MK, Terwel D, Kummer MP. Heneka MT, et al. J Neural Transm (Vienna). 2010 Aug;117(8):919-47. doi: 10.1007/s00702-010-0438-z. Epub 2010 Jul 15. J Neural Transm (Vienna). 2010. PMID: 20632195 Review. - Inflammation as a central mechanism in Alzheimer's disease.
Kinney JW, Bemiller SM, Murtishaw AS, Leisgang AM, Salazar AM, Lamb BT. Kinney JW, et al. Alzheimers Dement (N Y). 2018 Sep 6;4:575-590. doi: 10.1016/j.trci.2018.06.014. eCollection 2018. Alzheimers Dement (N Y). 2018. PMID: 30406177 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous