Staring, a novel E3 ubiquitin-protein ligase that targets syntaxin 1 for degradation - PubMed (original) (raw)

. 2002 Sep 20;277(38):35071-9.

doi: 10.1074/jbc.M203300200. Epub 2002 Jul 16.

Affiliations

• PMID: 12121982
• DOI: 10.1074/jbc.M203300200

Free article

Staring, a novel E3 ubiquitin-protein ligase that targets syntaxin 1 for degradation

Lih-Shen Chin et al. J Biol Chem. 2002.

Free article

Abstract

Syntaxin 1 is an essential component of the neurotransmitter release machinery, and regulation of syntaxin 1 expression levels is thought to contribute to the mechanism underlying learning and memory. However, the molecular events that control the degradation of syntaxin 1 remain undefined. Here we report the identification and characterization of a novel RING finger protein, Staring, that interacts with syntaxin 1. Staring is expressed throughout the brain, where it exists in both cytosolic and membrane-associated pools. Staring binds and recruits the brain-enriched E2 ubiquitin-conjugating enzyme UbcH8 to syntaxin 1 and facilitates the ubiquitination and proteasome-dependent degradation of syntaxin 1. These findings suggest that Staring is a novel E3 ubiquitin-protein ligase that targets syntaxin 1 for degradation by the ubiquitin-proteasome pathway.

PubMed Disclaimer

Similar articles

• Parkin functions as an E2-dependent ubiquitin- protein ligase and promotes the degradation of the synaptic vesicle-associated protein, CDCrel-1.
  Zhang Y, Gao J, Chung KK, Huang H, Dawson VL, Dawson TM. Zhang Y, et al. Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):13354-9. doi: 10.1073/pnas.240347797. Proc Natl Acad Sci U S A. 2000. PMID: 11078524 Free PMC article.
• Cloning and characterization of an androgen receptor N-terminal-interacting protein with ubiquitin-protein ligase activity.
  Beitel LK, Elhaji YA, Lumbroso R, Wing SS, Panet-Raymond V, Gottlieb B, Pinsky L, Trifiro MA. Beitel LK, et al. J Mol Endocrinol. 2002 Aug;29(1):41-60. doi: 10.1677/jme.0.0290041. J Mol Endocrinol. 2002. PMID: 12200228
• Regulation of synaptophysin degradation by mammalian homologues of seven in absentia.
  Wheeler TC, Chin LS, Li Y, Roudabush FL, Li L. Wheeler TC, et al. J Biol Chem. 2002 Mar 22;277(12):10273-82. doi: 10.1074/jbc.M107857200. Epub 2002 Jan 10. J Biol Chem. 2002. PMID: 11786535
• Part I: parkin-associated proteins and Parkinson's disease.
  Dev KK, van der Putten H, Sommer B, Rovelli G. Dev KK, et al. Neuropharmacology. 2003 Jul;45(1):1-13. doi: 10.1016/s0028-3908(02)00337-4. Neuropharmacology. 2003. PMID: 12814656 Review.
• U-box proteins as a new family of ubiquitin ligases.
  Hatakeyama S, Nakayama KI. Hatakeyama S, et al. Biochem Biophys Res Commun. 2003 Mar 21;302(4):635-45. doi: 10.1016/s0006-291x(03)00245-6. Biochem Biophys Res Commun. 2003. PMID: 12646216 Review.

Cited by

• Proteasome regulation of the chromodomain protein MRG-1 controls the balance between proliferative fate and differentiation in the C. elegans germ line.
  Gupta P, Leahul L, Wang X, Wang C, Bakos B, Jasper K, Hansen D. Gupta P, et al. Development. 2015 Jan 15;142(2):291-302. doi: 10.1242/dev.115147. Development. 2015. PMID: 25564623 Free PMC article.
• Proteolysis, synaptic plasticity and memory.
  Hegde AN. Hegde AN. Neurobiol Learn Mem. 2017 Feb;138:98-110. doi: 10.1016/j.nlm.2016.09.003. Epub 2016 Sep 7. Neurobiol Learn Mem. 2017. PMID: 27614141 Free PMC article. Review.
• Printor, a novel torsinA-interacting protein implicated in dystonia pathogenesis.
  Giles LM, Li L, Chin LS. Giles LM, et al. J Biol Chem. 2009 Aug 7;284(32):21765-75. doi: 10.1074/jbc.M109.004838. Epub 2009 Jun 17. J Biol Chem. 2009. PMID: 19535332 Free PMC article.
• Roles of ubiquitination at the synapse.
  Haas KF, Broadie K. Haas KF, et al. Biochim Biophys Acta. 2008 Aug;1779(8):495-506. doi: 10.1016/j.bbagrm.2007.12.010. Epub 2008 Jan 5. Biochim Biophys Acta. 2008. PMID: 18222124 Free PMC article. Review.
• Identification of ubiquitinated proteins in Arabidopsis.
  Manzano C, Abraham Z, López-Torrejón G, Del Pozo JC. Manzano C, et al. Plant Mol Biol. 2008 Sep;68(1-2):145-58. doi: 10.1007/s11103-008-9358-9. Epub 2008 Jun 6. Plant Mol Biol. 2008. PMID: 18535787

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • GlyGen glycoinformatics resource
  • IMEx Consortium

• Research Materials

  • NCI CPTC Antibody Characterization Program