The human dynamin-related protein OPA1 is anchored to the mitochondrial inner membrane facing the inter-membrane space - PubMed (original) (raw)

. 2002 Jul 17;523(1-3):171-6.

doi: 10.1016/s0014-5793(02)02985-x.

Laurent J Emorine, Eric Descoins, Laetitia Pelloquin, Laetitia Brichese, Nicole Gas, Emmanuelle Guillou, Cécile Delettre, Annie Valette, Christian P Hamel, Bernard Ducommun, Guy Lenaers, Pascale Belenguer

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• PMID: 12123827
• DOI: 10.1016/s0014-5793(02)02985-x

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The human dynamin-related protein OPA1 is anchored to the mitochondrial inner membrane facing the inter-membrane space

Aurélien Olichon et al. FEBS Lett. 2002.

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Abstract

Mutations in the OPA1 gene are associated with autosomal dominant optic atrophy. OPA1 encodes a dynamin-related protein orthologous to Msp1 of Schizosaccharomyces pombe and Mgm1p of Saccharomyces cerevisiae, both involved in mitochondrial morphology and genome maintenance. We present immuno-fluorescence and biochemical evidences showing that OPA1 resides in the mitochondria where it is imported through its highly basic amino-terminal extension. Proteolysis experiments indicate that OPA1 is present in the inter-membrane space and electron microscopy further localizes it close to the cristae. The strong association of OPA1 with membranes suggests its anchoring to the inner membrane.

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