In vivo, low-resistance, whole-cell recordings from neurons in the anaesthetized and awake mammalian brain - PubMed (original) (raw)
Comparative Study
doi: 10.1007/s00424-002-0831-z. Epub 2002 Apr 17.
Affiliations
- PMID: 12136268
- DOI: 10.1007/s00424-002-0831-z
Comparative Study
In vivo, low-resistance, whole-cell recordings from neurons in the anaesthetized and awake mammalian brain
Troy W Margrie et al. Pflugers Arch. 2002 Jul.
Abstract
A blind patch-clamp technique for in vivo whole-cell recordings in the intact brain is described. Recordings were obtained from various neuronal cell types located 100-5,000 microm from the cortical surface. Access resistance of recordings was as low as 10 M Omega but increased with recording depth and animal age. Recordings were remarkably stable and it was therefore possible to obtain whole-cell recordings in awake, head-fixed animals. The whole-cell configuration permitted rapid dialysis of cells with a calcium buffer. In most neurons very little ongoing action potential (AP) activity was observed and the spontaneous firing rates were up to 50-fold less than what has been reported by extracellular unit recordings. AP firing in the brain might therefore be far sparser than previously thought.
Similar articles
- Whole-cell recordings in freely moving rats.
Lee AK, Manns ID, Sakmann B, Brecht M. Lee AK, et al. Neuron. 2006 Aug 17;51(4):399-407. doi: 10.1016/j.neuron.2006.07.004. Neuron. 2006. PMID: 16908406 - Membrane properties and firing patterns of inferior colliculus neurons: an in vivo patch-clamp study in rodents.
Tan ML, Theeuwes HP, Feenstra L, Borst JG. Tan ML, et al. J Neurophysiol. 2007 Jul;98(1):443-53. doi: 10.1152/jn.01273.2006. Epub 2007 May 16. J Neurophysiol. 2007. PMID: 17507499 - Intrinsic excitability of cholinergic neurons in the rat parabigeminal nucleus.
Goddard CA, Knudsen EI, Huguenard JR. Goddard CA, et al. J Neurophysiol. 2007 Dec;98(6):3486-93. doi: 10.1152/jn.00960.2007. Epub 2007 Sep 26. J Neurophysiol. 2007. PMID: 17898138 - Genetic manipulation, whole-cell recordings and functional imaging of the sensorimotor cortex of behaving mice.
Petersen CC. Petersen CC. Acta Physiol (Oxf). 2009 Jan;195(1):91-9. doi: 10.1111/j.1748-1716.2008.01925.x. Epub 2008 Oct 28. Acta Physiol (Oxf). 2009. PMID: 18983448 Review. - [Basic micro-electrophysiologic techniques used in neurobiology].
Konopacki J, Bland BH, Dyck R. Konopacki J, et al. Postepy Hig Med Dosw. 2000;54(3):261-78. Postepy Hig Med Dosw. 2000. PMID: 10941261 Review. Polish.
Cited by
- Population diversity and function of hyperpolarization-activated current in olfactory bulb mitral cells.
Angelo K, Margrie TW. Angelo K, et al. Sci Rep. 2011;1:50. doi: 10.1038/srep00050. Epub 2011 Jul 29. Sci Rep. 2011. PMID: 22355569 Free PMC article. - The organization of two new cortical interneuronal circuits.
Jiang X, Wang G, Lee AJ, Stornetta RL, Zhu JJ. Jiang X, et al. Nat Neurosci. 2013 Feb;16(2):210-8. doi: 10.1038/nn.3305. Epub 2013 Jan 13. Nat Neurosci. 2013. PMID: 23313910 Free PMC article. - Layer 4 fast-spiking interneurons filter thalamocortical signals during active somatosensation.
Yu J, Gutnisky DA, Hires SA, Svoboda K. Yu J, et al. Nat Neurosci. 2016 Dec;19(12):1647-1657. doi: 10.1038/nn.4412. Epub 2016 Oct 17. Nat Neurosci. 2016. PMID: 27749825 - In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives.
Noguchi A, Ikegaya Y, Matsumoto N. Noguchi A, et al. Sensors (Basel). 2021 Feb 19;21(4):1448. doi: 10.3390/s21041448. Sensors (Basel). 2021. PMID: 33669656 Free PMC article. Review. - Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience.
Tao C, Zhang G, Xiong Y, Zhou Y. Tao C, et al. Front Neural Circuits. 2015 May 22;9:23. doi: 10.3389/fncir.2015.00023. eCollection 2015. Front Neural Circuits. 2015. PMID: 26052270 Free PMC article. Review.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources