Quadrupole ion-trap mass spectrometry to locate fatty acids on lipid A from Gram-negative bacteria - PubMed (original) (raw)
Quadrupole ion-trap mass spectrometry to locate fatty acids on lipid A from Gram-negative bacteria
Anders Kussak et al. Anal Biochem. 2002.
Abstract
The structure of lipid A released by mild acid hydrolysis from lipopolysaccharide from two strains of Shigella flexneri with different degrees of acylation was characterized using electrospray ionization (ESI) and ion-trap mass spectrometry. The lipid A was analyzed underivatized with ESI in negative-ion mode. With multiple stages of fragmentation (MS(n)), both the degree of acylation and the positions of the fatty acids on the disaccharide backbone could be determined. It was possible to determine the degree of acylation by the MS(n) technique, where in each MS stage the parent ion was an ion where one fatty acid had been eliminated. One way to determine the location of the fatty acids was by identifying cross-ring fragments of the reducing sugar from parent ions containing different numbers of fatty acids. Another was by identifying a possible charge-driven release of fatty acids situated close to a phosphate group. The fatty acids were otherwise eliminated by a charge-remote fragmentation mechanism. The combined data show the usefulness of ion-trap mass spectrometers for this type of analysis.
Similar articles
- Structural investigation by tandem mass spectrometry analysis of a heterogeneous mixture of Lipid An isolated from the lipopolysaccharide of Aeromonas hydrophila SJ-55Ra.
Almostafa M, Fridgen TD, Banoub JH. Almostafa M, et al. Rapid Commun Mass Spectrom. 2018 Feb 15;32(3):167-183. doi: 10.1002/rcm.8017. Rapid Commun Mass Spectrom. 2018. PMID: 29065225 - Occurrence of an unusual hopanoid-containing lipid A among lipopolysaccharides from Bradyrhizobium species.
Komaniecka I, Choma A, Mazur A, Duda KA, Lindner B, Schwudke D, Holst O. Komaniecka I, et al. J Biol Chem. 2014 Dec 19;289(51):35644-55. doi: 10.1074/jbc.M114.614529. Epub 2014 Nov 3. J Biol Chem. 2014. PMID: 25371196 Free PMC article. - Mass spectrometry advances in lipidomica: collision-induced decomposition of Kdo2-lipid A.
Murphy RC, Raetz CR, Reynolds CM, Barkley RM. Murphy RC, et al. Prostaglandins Other Lipid Mediat. 2005 Sep;77(1-4):131-40. doi: 10.1016/j.prostaglandins.2004.09.004. Prostaglandins Other Lipid Mediat. 2005. PMID: 16099398 Free PMC article. Review.
Cited by
- Novel modification of lipid A of Francisella tularensis.
Phillips NJ, Schilling B, McLendon MK, Apicella MA, Gibson BW. Phillips NJ, et al. Infect Immun. 2004 Sep;72(9):5340-8. doi: 10.1128/IAI.72.9.5340-5348.2004. Infect Immun. 2004. PMID: 15322031 Free PMC article. - LipidA-IDER to Explore the Global Lipid A Repertoire of Drug-Resistant Gram-Negative Bacteria.
Guan XL, Loh JY, Lizwan M, Chan SCM, Kwan JMC, Lim TP, Koh TH, Hsu LY, Lee BTK. Guan XL, et al. Anal Chem. 2023 Jan 17;95(2):602-611. doi: 10.1021/acs.analchem.1c03566. Epub 2023 Jan 4. Anal Chem. 2023. PMID: 36599414 Free PMC article. - Structural characterization of phosphoethanolamine-modified lipid A from probiotic Escherichia coli strain Nissle 1917.
Jo SH, Park HG, Song WS, Kim SM, Kim EJ, Yang YH, Kim JS, Kim BG, Kim YG. Jo SH, et al. RSC Adv. 2019 Jun 25;9(34):19762-19771. doi: 10.1039/c9ra02375e. eCollection 2019 Jun 19. RSC Adv. 2019. PMID: 35519361 Free PMC article. - Study on the CID Fragmentation Pathways of Deprotonated 4'-Monophosphoryl Lipid A.
Aissa I, Kilár A, Dörnyei Á. Aissa I, et al. Molecules. 2021 Oct 1;26(19):5961. doi: 10.3390/molecules26195961. Molecules. 2021. PMID: 34641505 Free PMC article. - A Multimodal System for Lipid A Structural Analysis from a Single Colony.
Yang H, O'Keefe I, Smith RD, Sumner KP, Sherman ME, Goodlett DR, Sweet CR, Ernst RK. Yang H, et al. Anal Chem. 2024 Aug 16;96(34):13838-45. doi: 10.1021/acs.analchem.4c01566. Online ahead of print. Anal Chem. 2024. PMID: 39149983 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous