Sphingosylphosphorylcholine is a novel messenger for Rho-kinase-mediated Ca2+ sensitization in the bovine cerebral artery: unimportant role for protein kinase C - PubMed (original) (raw)
. 2002 Jul 26;91(2):112-9.
doi: 10.1161/01.res.0000026057.13161.42.
Shiro Kashiwagi, Masafumi Sato, Saori Miwa, Fumiaki Nakao, Tetsu Kurokawa, Natsuko Todoroki-Ikeda, Kimiko Mogami, Yoichi Mizukami, Shinichi Kuriyama, Kyousuke Haze, Michiyasu Suzuki, Sei Kobayashi
Affiliations
- PMID: 12142343
- DOI: 10.1161/01.res.0000026057.13161.42
Sphingosylphosphorylcholine is a novel messenger for Rho-kinase-mediated Ca2+ sensitization in the bovine cerebral artery: unimportant role for protein kinase C
Satoshi Shirao et al. Circ Res. 2002.
Abstract
Although recent investigations have suggested that a Rho-kinase-mediated Ca2+ sensitization of vascular smooth muscle contraction plays a critical role in the pathogenesis of cerebral and coronary vasospasm, the upstream of this signal transduction has not been elucidated. In addition, the involvement of protein kinase C (PKC) may also be related to cerebral vasospasm. We recently reported that sphingosylphosphorylcholine (SPC), a sphingolipid, induces Rho-kinase-mediated Ca2+ sensitization in pig coronary arteries. The purpose of this present study was to examine the possible mediation of SPC in Ca2+ sensitization of the bovine middle cerebral artery (MCA) and the relation to signal transduction pathways mediated by Rho-kinase and PKC. In intact MCA, SPC induced a concentration-dependent (EC50=3.0 micromol/L) contraction, without [Ca2+]i elevation. In membrane-permeabilized MCA, SPC induced Ca2+ sensitization even in the absence of added GTP, which is required for activation of G-proteins coupled to membrane receptors. The SPC-induced Ca2+ sensitization was blocked by a Rho-kinase inhibitor (Y-27632) and a dominant-negative Rho-kinase, but not by a pseudosubstrate peptide for conventional PKC, which abolished the Ca2+-independent contraction induced by phorbol ester. In contrast, phorbol ester-induced Ca2+ sensitization was resistant to a Rho-kinase inhibitor and a dominant-negative Rho-kinase. In primary cultured vascular smooth muscle cells, SPC induced the translocation of cytosolic Rho-kinase to the cell membrane. We propose that SPC is a novel messenger for Rho-kinase-mediated Ca2+ sensitization of cerebral arterial smooth muscle and, therefore, may play a pivotal role in the pathogenesis of abnormal contraction of the cerebral artery such as vasospasm. The SPC/Rho-kinase pathway functions independently of the PKC pathway.
Comment in
- New roads leading to Ca2+ sensitization.
Somlyo AV. Somlyo AV. Circ Res. 2002 Jul 26;91(2):83-4. doi: 10.1161/01.res.0000028341.63905.91. Circ Res. 2002. PMID: 12142337 No abstract available.
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