Biosynthesis of the enediyne antitumor antibiotic C-1027 - PubMed (original) (raw)

. 2002 Aug 16;297(5584):1170-3.

doi: 10.1126/science.1072110.

Affiliations

• PMID: 12183628
• DOI: 10.1126/science.1072110

Biosynthesis of the enediyne antitumor antibiotic C-1027

Wen Liu et al. Science. 2002.

Abstract

C-1027 is a potent antitumor agent with a previously undescribed molecular architecture and mode of action. Cloning and characterization of the 85-kilobase C-1027 biosynthesis gene cluster from Streptomyces globisporus revealed (i) an iterative type I polyketide synthase that is distinct from any bacterial polyketide synthases known to date, (ii) a general polyketide pathway for the biosynthesis of both the 9- and 10-membered enediyne antibiotics, and (iii) a convergent biosynthetic strategy for the C-1027 chromophore from four building blocks. Manipulation of genes governing C-1027 biosynthesis allowed us to produce an enediyne compound in a predicted manner.

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