Low doses of the endocrine disruptor bisphenol-A and the native hormone 17beta-estradiol rapidly activate transcription factor CREB - PubMed (original) (raw)

. 2002 Oct;16(12):1671-3.

doi: 10.1096/fj.02-0313fje. Epub 2002 Aug 21.

Affiliations

• PMID: 12207000
• DOI: 10.1096/fj.02-0313fje

Low doses of the endocrine disruptor bisphenol-A and the native hormone 17beta-estradiol rapidly activate transcription factor CREB

Ivan Quesada et al. FASEB J. 2002 Oct.

Abstract

Endocrine-disrupting chemicals (EDCs) are hormone-like agents present in the environment that alter the endocrine system of wildlife and humans. Most EDCs have potencies far below those of the natural hormone 17beta-E2 when acting through the classic estrogen receptors (ERs). Here, we show that the environmental estrogen Bisphenol-A and the native hormone 17beta-E2 activate the transcription factor, cAMP-responsive element binding protein (CREB) with the same potency. Phosphorylated CREB (P-CREB) was increased after only a 5-minute application of either BPA or 17beta-E2 in a calcium-dependent manner. The effect was reproduced by the membrane-impermeable molecule E2 conjugated to horseradish peroxidase (E-HRP). The increase in P-CREB was not modified by the anti-estrogen ICI 182,780. Therefore, low-dose of BPA activates the transcription factor CREB via an alternative mechanism, involving a non-classical membrane estrogen receptor. Because these effects are elicited at concentrations as low as 10(-9) M, this observation is of environmental and public health relevance.

PubMed Disclaimer

Similar articles

• Low doses of bisphenol A promote human seminoma cell proliferation by activating PKA and PKG via a membrane G-protein-coupled estrogen receptor.
  Bouskine A, Nebout M, Brücker-Davis F, Benahmed M, Fenichel P. Bouskine A, et al. Environ Health Perspect. 2009 Jul;117(7):1053-8. doi: 10.1289/ehp.0800367. Epub 2009 Feb 11. Environ Health Perspect. 2009. PMID: 19654912 Free PMC article.
• Rapid actions of estradiol on cyclic amp response-element binding protein phosphorylation in dorsal root ganglion neurons.
  Purves-Tyson TD, Keast JR. Purves-Tyson TD, et al. Neuroscience. 2004;129(3):629-37. doi: 10.1016/j.neuroscience.2004.08.019. Neuroscience. 2004. PMID: 15541884
• Estrogen and xenoestrogen actions on endocrine pancreas: from ion channel modulation to activation of nuclear function.
  Nadal A, Ropero AB, Fuentes E, Soria B, Ripoll C. Nadal A, et al. Steroids. 2004 Aug;69(8-9):531-6. doi: 10.1016/j.steroids.2004.05.010. Steroids. 2004. PMID: 15288765 Review.
• Low doses of bisphenol A and diethylstilbestrol impair Ca2+ signals in pancreatic alpha-cells through a nonclassical membrane estrogen receptor within intact islets of Langerhans.
  Alonso-Magdalena P, Laribi O, Ropero AB, Fuentes E, Ripoll C, Soria B, Nadal A. Alonso-Magdalena P, et al. Environ Health Perspect. 2005 Aug;113(8):969-77. doi: 10.1289/ehp.8002. Environ Health Perspect. 2005. PMID: 16079065 Free PMC article.
• Bisphenol-A acts as a potent estrogen via non-classical estrogen triggered pathways.
  Alonso-Magdalena P, Ropero AB, Soriano S, García-Arévalo M, Ripoll C, Fuentes E, Quesada I, Nadal Á. Alonso-Magdalena P, et al. Mol Cell Endocrinol. 2012 May 22;355(2):201-7. doi: 10.1016/j.mce.2011.12.012. Epub 2011 Dec 31. Mol Cell Endocrinol. 2012. PMID: 22227557 Review.

Cited by

• The hijacking of cellular signaling and the diabetes epidemic: mechanisms of environmental disruption of insulin action and glucose homeostasis.
  Sargis RM. Sargis RM. Diabetes Metab J. 2014 Feb;38(1):13-24. doi: 10.4093/dmj.2014.38.1.13. Diabetes Metab J. 2014. PMID: 24627823 Free PMC article. Review.
• Timing of Exposure and Bisphenol-A: Implications for Diabetes Development.
  Tudurí E, Marroqui L, Dos Santos RS, Quesada I, Fuentes E, Alonso-Magdalena P. Tudurí E, et al. Front Endocrinol (Lausanne). 2018 Oct 31;9:648. doi: 10.3389/fendo.2018.00648. eCollection 2018. Front Endocrinol (Lausanne). 2018. PMID: 30429829 Free PMC article. Review.
• Oncogenic Potential of Bisphenol A and Common Environmental Contaminants in Human Mammary Epithelial Cells.
  Nair VA, Valo S, Peltomäki P, Bajbouj K, Abdel-Rahman WM. Nair VA, et al. Int J Mol Sci. 2020 May 25;21(10):3735. doi: 10.3390/ijms21103735. Int J Mol Sci. 2020. PMID: 32466334 Free PMC article.
• Mice lacking membrane estrogen receptor 1 are protected from reproductive pathologies resulting from developmental estrogen exposure†.
  Nanjappa MK, Medrano TI, Mesa AM, Ortega MT, Caldo PD, Mao J, Kinkade JA, Levin ER, Rosenfeld CS, Cooke PS. Nanjappa MK, et al. Biol Reprod. 2019 Aug 1;101(2):392-404. doi: 10.1093/biolre/ioz090. Biol Reprod. 2019. PMID: 31141131 Free PMC article.
• Bisphenol A: Targeting metabolic tissues.
  Chevalier N, Fénichel P. Chevalier N, et al. Rev Endocr Metab Disord. 2015 Dec;16(4):299-309. doi: 10.1007/s11154-016-9333-8. Rev Endocr Metab Disord. 2015. PMID: 26820262 Review.

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley