The surface of lipid droplets is a phospholipid monolayer with a unique Fatty Acid composition - PubMed (original) (raw)
. 2002 Nov 15;277(46):44507-12.
doi: 10.1074/jbc.M207712200. Epub 2002 Sep 6.
Affiliations
- PMID: 12221100
- DOI: 10.1074/jbc.M207712200
Free article
The surface of lipid droplets is a phospholipid monolayer with a unique Fatty Acid composition
Kumi Tauchi-Sato et al. J Biol Chem. 2002.
Free article
Abstract
We found that caveolin-2 is targeted to the surface of lipid droplets (Fujimoto, T., Kogo, H., Ishiguro, K., Tauchi, K., and Nomura, R. (2001) J. Cell Biol. 152, 1079-1085) and hypothesized that the lipid droplet surface is a kind of membrane. To elucidate the characteristics of the lipid droplet surface, we isolated lipid droplets from HepG2 cells and analyzed them by cryoelectron microscopy and by mass spectrometry. By use of cryoelectron microscopy at the stage temperature of 4.2 K, the lipid droplet surface was observed as a single line without any fixation or staining, indicating the presence of a single layer of phospholipids. This result appeared consistent with the hypothesis that the lipid droplet surface is derived from the cytoplasmic leaflet of the endoplasmic reticulum membrane and may be continuous to it. However, mass spectrometry revealed that the fatty acid composition of phosphatidylcholine and lysophosphatidylcholine in lipid droplets is different from that of the rough endoplasmic reticulum. The ample presence of free cholesterol in lipid droplets also suggests that their surface is differentiated from the bulk endoplasmic reticulum membrane. On the other hand, although caveolin-2beta and adipose differentiation-related protein, both localizing in lipid droplets, were enriched in the low density floating fraction, the fatty acid composition of the fraction was distinct from lipid droplets. Collectively, the result indicates that the lipid droplet surface is a hemi-membrane or a phospholipid monolayer containing cholesterol but is compositionally different from the endoplasmic reticulum membrane or the sphingolipid/cholesterol-rich microdomain.
Similar articles
- Changes in the phospholipid fatty acid composition of the lipid droplet during the differentiation of 3T3-L1 adipocytes.
Arisawa K, Ichi I, Yasukawa Y, Sone Y, Fujiwara Y. Arisawa K, et al. J Biochem. 2013 Sep;154(3):281-9. doi: 10.1093/jb/mvt051. Epub 2013 Jun 12. J Biochem. 2013. PMID: 23760554 - Saturated fatty acid in the phospholipid monolayer contributes to the formation of large lipid droplets.
Arisawa K, Mitsudome H, Yoshida K, Sugimoto S, Ishikawa T, Fujiwara Y, Ichi I. Arisawa K, et al. Biochem Biophys Res Commun. 2016 Nov 25;480(4):641-647. doi: 10.1016/j.bbrc.2016.10.109. Epub 2016 Oct 27. Biochem Biophys Res Commun. 2016. PMID: 27983976 - Lipid droplet analysis in caveolin-deficient adipocytes: alterations in surface phospholipid composition and maturation defects.
Blouin CM, Le Lay S, Eberl A, Köfeler HC, Guerrera IC, Klein C, Le Liepvre X, Lasnier F, Bourron O, Gautier JF, Ferré P, Hajduch E, Dugail I. Blouin CM, et al. J Lipid Res. 2010 May;51(5):945-56. doi: 10.1194/jlr.M001016. Epub 2009 Nov 10. J Lipid Res. 2010. PMID: 19965594 Free PMC article. - Phospholipids and lipid droplets.
Penno A, Hackenbroich G, Thiele C. Penno A, et al. Biochim Biophys Acta. 2013 Mar;1831(3):589-94. doi: 10.1016/j.bbalip.2012.12.001. Epub 2012 Dec 12. Biochim Biophys Acta. 2013. PMID: 23246574 Review. - Fat Cell and Fatty Acid Turnover in Obesity.
Engin A. Engin A. Adv Exp Med Biol. 2017;960:135-160. doi: 10.1007/978-3-319-48382-5_6. Adv Exp Med Biol. 2017. PMID: 28585198 Review.
Cited by
- Antioxidant and antiadipogenic activities of galkeun-tang, a traditional korean herbal formula.
Jeong SJ, Yoo SR, Kim OS, Seo CS, Shin HK. Jeong SJ, et al. Evid Based Complement Alternat Med. 2014;2014:763494. doi: 10.1155/2014/763494. Epub 2014 Dec 10. Evid Based Complement Alternat Med. 2014. PMID: 25574183 Free PMC article. - Specialized Cortex Glial Cells Accumulate Lipid Droplets in Drosophila melanogaster.
Kis V, Barti B, Lippai M, Sass M. Kis V, et al. PLoS One. 2015 Jul 6;10(7):e0131250. doi: 10.1371/journal.pone.0131250. eCollection 2015. PLoS One. 2015. PMID: 26148013 Free PMC article. - Label-free imaging and analysis of the effects of lipolysis products on primary hepatocytes.
Schie IW, Wu J, Weeks T, Zern MA, Rutledge JC, Huser T. Schie IW, et al. J Biophotonics. 2011 Jun;4(6):425-34. doi: 10.1002/jbio.201000086. Epub 2010 Sep 28. J Biophotonics. 2011. PMID: 20878906 Free PMC article. - High-resolution proton NMR measures mobile lipids associated with Triton-resistant membrane domains in haematopoietic K562 cells lacking or expressing caveolin-1.
Ferretti A, Knijn A, Raggi C, Sargiacomo M. Ferretti A, et al. Eur Biophys J. 2003 May;32(2):83-95. doi: 10.1007/s00249-002-0273-8. Epub 2003 Jan 28. Eur Biophys J. 2003. PMID: 12734696 - Roles and origins of leukocyte lipid bodies: proteomic and ultrastructural studies.
Wan HC, Melo RC, Jin Z, Dvorak AM, Weller PF. Wan HC, et al. FASEB J. 2007 Jan;21(1):167-78. doi: 10.1096/fj.06-6711com. Epub 2006 Nov 29. FASEB J. 2007. PMID: 17135363 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials