Local and Systemic Biosynthesis of Salicylic Acid in Infected Cucumber Plants - PubMed (original) (raw)

Local and Systemic Biosynthesis of Salicylic Acid in Infected Cucumber Plants

P. Meuwly et al. Plant Physiol. 1995 Nov.

Abstract

Radiolabeling studies showed that salicylic acid (SA), an essential component in the signal transduction pathway leading to systemic acquired resistance, is synthesized from phenylalanine (Phe) and benzoic acid in cucumber (Cucumis sativus L.) plants inoculated with pathogens. Leaf discs from plants inoculated with either tobacco necrosis virus or Pseudomonas lachrymans incorporated more [14C]Phe into [14C]SA than mock-inoculated controls. The identity of SA was confirmed by gas chromatography-mass spectrometry. No reduction in specific activity of [14C]SA was observed for either free or bound SA between control and infected plants after feeding [14C]Phe. A specific inhibitor of Phe ammonia-lyase, 2-aminoindan-2-phosphonic acid, completely inhibited the incorporation of [14C]Phe into [14C]SA, although plants treated with 2-aminoindan-2-phosphonic acid could still produce [14C]SA from [14C]benzoic acid. Biosynthesis of SA in tissue inoculated with tobacco necrosis virus followed a transient pattern with the highest induction occurring 72 h postinoculation. Uninfected tissues from an infected plant synthesized de novo more SA than did controls. This suggests the involvement of a systemic signal triggering SA synthesis in tissue distant from the site of infection that display systemic acquired resistance.

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References

    1. Plant Physiol. 1993 Oct;103(2):323-328 - PubMed
    1. Plant Physiol. 1995 Aug;108(4):1379-1385 - PubMed
    1. Science. 1990 Nov 16;250(4983):1002-4 - PubMed
    1. Anal Biochem. 1993 Nov 1;214(2):500-5 - PubMed
    1. Science. 1990 Nov 16;250(4983):1004-6 - PubMed

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