Modulation of hippocampal glutamatergic transmission by ATP is dependent on adenosine a(1) receptors - PubMed (original) (raw)
Modulation of hippocampal glutamatergic transmission by ATP is dependent on adenosine a(1) receptors
Susan A Masino et al. J Pharmacol Exp Ther. 2002 Oct.
Abstract
Excitatory glutamatergic synapses in the hippocampal CA1 region of rats are potently inhibited by purines, including adenosine, ATP, and ATP analogs. Adenosine A(1) receptors are known to mediate at least part of the response to adenine nucleotides, either because adenine nucleotides activate A(1) receptors directly, or activate them secondarily upon the nucleotides' conversion to adenosine. In the present studies, the inhibitory effects of adenosine, ATP, the purportedly stable ATP analog adenosine-5'-O-(3-thio)triphosphate (ATPgammaS), and cyclic AMP were examined in mice with a null mutation in the adenosine A(1) receptor gene. ATPgammaS displaced the binding of A(1)-selective ligands to intact brain sections and brain homogenates from adenosine A(1) receptor wild-type animals. In homogenates, but not in intact brain sections, this displacement was abolished by adenosine deaminase. In hippocampal slices from wild-type mice, purines abolished synaptic responses, but slices from mice lacking functional A(1) receptors showed no synaptic modulation by adenosine, ATP, cAMP, or ATPgammaS. In slices from heterozygous mice the dose-response curve for both adenosine and ATP was shifted to the right. In all cases, inhibition of synaptic responses by purines could be blocked by prior treatment with the competitive adenosine A(1) receptor antagonist 8-cyclopentyltheophylline. Taken together, these results show that even supposedly stable adenine nucleotides are rapidly converted to adenosine at sites close to the A(1) receptor, and that inhibition of synaptic transmission by purine nucleotides is mediated exclusively by A(1) receptors.
Similar articles
- Adenosine A(2A) receptor facilitation of hippocampal synaptic transmission is dependent on tonic A(1) receptor inhibition.
Lopes LV, Cunha RA, Kull B, Fredholm BB, Ribeiro JA. Lopes LV, et al. Neuroscience. 2002;112(2):319-29. doi: 10.1016/s0306-4522(02)00080-5. Neuroscience. 2002. PMID: 12044450 - Preferential activation of excitatory adenosine receptors at rat hippocampal and neuromuscular synapses by adenosine formed from released adenine nucleotides.
Cunha RA, Correia-de-Sá P, Sebastião AM, Ribeiro JA. Cunha RA, et al. Br J Pharmacol. 1996 Sep;119(2):253-60. doi: 10.1111/j.1476-5381.1996.tb15979.x. Br J Pharmacol. 1996. PMID: 8886406 Free PMC article. - Inhibition by ATP of hippocampal synaptic transmission requires localized extracellular catabolism by ecto-nucleotidases into adenosine and channeling to adenosine A1 receptors.
Cunha RA, Sebastião AM, Ribeiro JA. Cunha RA, et al. J Neurosci. 1998 Mar 15;18(6):1987-95. doi: 10.1523/JNEUROSCI.18-06-01987.1998. J Neurosci. 1998. PMID: 9482785 Free PMC article. Review. - Regulation of muscarinic acetylcholine receptor-mediated synaptic responses by adenosine receptors in the rat hippocampus.
Morton RA, Davies CH. Morton RA, et al. J Physiol. 1997 Jul 1;502 ( Pt 1)(Pt 1):75-90. doi: 10.1111/j.1469-7793.1997.075bl.x. J Physiol. 1997. PMID: 9234198 Free PMC article. - Purinergic regulation of acetylcholine release.
Ribeiro JA, Cunha RA, Correia-de-Sá P, Sebastião AM. Ribeiro JA, et al. Prog Brain Res. 1996;109:231-41. doi: 10.1016/s0079-6123(08)62107-x. Prog Brain Res. 1996. PMID: 9009712 Review.
Cited by
- Homo- and heteroexchange of adenine nucleotides and nucleosides in rat hippocampal slices by the nucleoside transport system.
Sperlágh B, Szabó G, Erdélyi F, Baranyi M, Vizi ES. Sperlágh B, et al. Br J Pharmacol. 2003 Jun;139(3):623-33. doi: 10.1038/sj.bjp.0705285. Br J Pharmacol. 2003. PMID: 12788822 Free PMC article. - ATP inhibits NMDA receptors after heterologous expression and in cultured hippocampal neurons and attenuates NMDA-mediated neurotoxicity.
Ortinau S, Laube B, Zimmermann H. Ortinau S, et al. J Neurosci. 2003 Jun 15;23(12):4996-5003. doi: 10.1523/JNEUROSCI.23-12-04996.2003. J Neurosci. 2003. PMID: 12832522 Free PMC article. - Dual presynaptic control by ATP of glutamate release via facilitatory P2X1, P2X2/3, and P2X3 and inhibitory P2Y1, P2Y2, and/or P2Y4 receptors in the rat hippocampus.
Rodrigues RJ, Almeida T, Richardson PJ, Oliveira CR, Cunha RA. Rodrigues RJ, et al. J Neurosci. 2005 Jul 6;25(27):6286-95. doi: 10.1523/JNEUROSCI.0628-05.2005. J Neurosci. 2005. PMID: 16000618 Free PMC article. - P2 receptor-mediated modulation of neurotransmitter release-an update.
Sperlágh B, Heinrich A, Csölle C. Sperlágh B, et al. Purinergic Signal. 2007 Sep;3(4):269-84. doi: 10.1007/s11302-007-9080-0. Epub 2007 Oct 9. Purinergic Signal. 2007. PMID: 18404441 Free PMC article. - Roles of purines in synaptic modulation evoked by hypercapnia in isolated spinal cord of neonatal rat in vitro.
Otsuguro K, Ban M, Ohta T, Ito S. Otsuguro K, et al. Br J Pharmacol. 2009 Apr;156(7):1167-77. doi: 10.1111/j.1476-5381.2009.00118.x. Br J Pharmacol. 2009. PMID: 19378379 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous