An initiation site for meiotic crossing-over and gene conversion in the mouse - PubMed (original) (raw)
doi: 10.1038/ng990. Epub 2002 Sep 16.
Affiliations
- PMID: 12244318
- DOI: 10.1038/ng990
An initiation site for meiotic crossing-over and gene conversion in the mouse
Hélène Guillon et al. Nat Genet. 2002 Oct.
Abstract
During meiosis, the reductional segregation of homologous chromosomes at the first meiotic division requires reciprocal exchange (crossing over) between homologs. The number of crossovers is tightly regulated (one to two per homolog in mice), and their distribution in the genome is not random-recombination 'hot' and 'cold' regions can be identified. We developed a molecular assay to study these events directly in mouse germ cells. This analysis was developed with reference to the proteosome subunit beta type 9 (Psmb9, previously called Lmp2) hot-spot region identified through genetic analysis. Here we show that this hot spot is an initiation site of meiotic recombination on the basis of two observations: (i) crossover density is maximal in an interval of 210 bp and decreases on both sides of this region; (ii) a high frequency of gene conversion is found in the region of highest crossover density. We then used this strategy to carry out the first temporal analysis of meiotic recombination in mouse spermatogenesis and demonstrate that crossover events occur during the pachytene stage of meiotic prophase.
Similar articles
- Reciprocal crossover asymmetry and meiotic drive in a human recombination hot spot.
Jeffreys AJ, Neumann R. Jeffreys AJ, et al. Nat Genet. 2002 Jul;31(3):267-71. doi: 10.1038/ng910. Epub 2002 Jun 24. Nat Genet. 2002. PMID: 12089523 - Intense and highly localized gene conversion activity in human meiotic crossover hot spots.
Jeffreys AJ, May CA. Jeffreys AJ, et al. Nat Genet. 2004 Feb;36(2):151-6. doi: 10.1038/ng1287. Epub 2004 Jan 4. Nat Genet. 2004. PMID: 14704667 - Meiotic crossing-over in the regions of homology between homologous chromosomes V.
Shubochkina EA, Nielsen TL, Nilsson-Tillgren T. Shubochkina EA, et al. Yeast. 2001 Sep 15;18(12):1173-83. doi: 10.1002/yea.759. Yeast. 2001. PMID: 11536338 - Branching out: meiotic recombination and its regulation.
Cromie GA, Smith GR. Cromie GA, et al. Trends Cell Biol. 2007 Sep;17(9):448-55. doi: 10.1016/j.tcb.2007.07.007. Epub 2007 Aug 24. Trends Cell Biol. 2007. PMID: 17719784 Review. - Parallel detection of crossovers and noncrossovers in mouse germ cells.
Baudat F, de Massy B. Baudat F, et al. Methods Mol Biol. 2009;557:305-22. doi: 10.1007/978-1-59745-527-5_19. Methods Mol Biol. 2009. PMID: 19799190 Review.
Cited by
- Genetic dissection of crossover mutants defines discrete intermediates in mouse meiosis.
Premkumar T, Paniker L, Kang R, Biot M, Humphrey E, Destain H, Ferranti I, Okulate I, Nguyen H, Kilaru V, Frasca M, Chakraborty P, Cole F. Premkumar T, et al. Mol Cell. 2023 Aug 17;83(16):2941-2958.e7. doi: 10.1016/j.molcel.2023.07.022. Mol Cell. 2023. PMID: 37595556 Free PMC article. - Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids.
Gergelits V, Parvanov E, Simecek P, Forejt J. Gergelits V, et al. Genetics. 2021 Mar 3;217(1):1-14. doi: 10.1093/genetics/iyaa013. Genetics. 2021. PMID: 33683354 Free PMC article. - Two telomeric ends of acrocentric chromosome play distinct roles in homologous chromosome synapsis in the fetal mouse oocyte.
Kazemi P, Taketo T. Kazemi P, et al. Chromosoma. 2021 Mar;130(1):41-52. doi: 10.1007/s00412-021-00752-1. Epub 2021 Jan 25. Chromosoma. 2021. PMID: 33492414 - Identification of Y chromosome markers in the eastern three-lined skink (Bassiana duperreyi) using in silico whole genome subtraction.
Dissanayake DSB, Holleley CE, Hill LK, O'Meally D, Deakin JE, Georges A. Dissanayake DSB, et al. BMC Genomics. 2020 Sep 29;21(1):667. doi: 10.1186/s12864-020-07071-2. BMC Genomics. 2020. PMID: 32993477 Free PMC article. - TH2BS11ph histone mark is enriched in the unsynapsed axes of the XY body and predominantly associates with H3K4me3-containing genomic regions in mammalian spermatocytes.
Mahadevan IA, Pentakota S, Roy R, Bhaduri U, Satyanarayana Rao MR. Mahadevan IA, et al. Epigenetics Chromatin. 2019 Sep 7;12(1):53. doi: 10.1186/s13072-019-0300-y. Epigenetics Chromatin. 2019. PMID: 31493790 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources