Phylogenetic evidence for frequent positive selection and recombination in the meningococcal surface antigen PorB - PubMed (original) (raw)
Phylogenetic evidence for frequent positive selection and recombination in the meningococcal surface antigen PorB
Rachel Urwin et al. Mol Biol Evol. 2002 Oct.
Abstract
Previous estimates of rates of synonymous (d(S)) and nonsynonymous (d(N)) substitution among Neisseria meningitidis gene sequences suggested that the surface loops of the variable outer membrane protein PorB were under only weak selection pressure from the host immune response. These findings were consistent with studies indicating that PorB variants were not always protective in immunological and microbiological assays and questioned the suitability of this protein as a vaccine component. PorB, which is expressed at high levels on the surface of the meningococcus, has been implicated in mechanisms of pathogenesis and has also been used as a typing target in epidemiological investigations. In this work, using more precise estimates of selection pressures and recombination rates, we have shown that some residues in the surface loops of PorB are under very strong positive selection, as great as that observed in human immunodeficiency virus-1 surface glycoproteins, whereas amino acids within the loops and the membrane-spanning regions of the protein are under purifying selection, presumably because of structural constraints. Congruence tests showed that recombination occurred at a rate that was not sufficient to erase all phylogenetic similarity and did not greatly bias selection analysis. Homology models of PorB structure indicated that many strongly selected sites encoded residues that were predicted to be exposed to host immune responses, implying that this protein is under strong immune selection and requires further examination as a potential vaccine candidate. These data show that phylogenetic inference can be used to complement immunological and biochemical data in the choice of vaccine candidates.
Similar articles
- Structural and evolutionary inference from molecular variation in Neisseria porins.
Derrick JP, Urwin R, Suker J, Feavers IM, Maiden MC. Derrick JP, et al. Infect Immun. 1999 May;67(5):2406-13. doi: 10.1128/IAI.67.5.2406-2413.1999. Infect Immun. 1999. PMID: 10225902 Free PMC article. - Variation in the Neisseria lactamica porin, and its relationship to meningococcal PorB.
Bennett JS, Callaghan MJ, Derrick JP, Maiden MCJ. Bennett JS, et al. Microbiology (Reading). 2008 May;154(Pt 5):1525-1534. doi: 10.1099/mic.0.2007/015479-0. Microbiology (Reading). 2008. PMID: 18451061 Free PMC article. - Distribution of surface protein variants among hyperinvasive meningococci: implications for vaccine design.
Urwin R, Russell JE, Thompson EA, Holmes EC, Feavers IM, Maiden MC. Urwin R, et al. Infect Immun. 2004 Oct;72(10):5955-62. doi: 10.1128/IAI.72.10.5955-5962.2004. Infect Immun. 2004. PMID: 15385499 Free PMC article. - The Impact of Nucleotide Sequence Analysis on Meningococcal Vaccine Development and Assessment.
Maiden MCJ. Maiden MCJ. Front Immunol. 2019 Jan 15;9:3151. doi: 10.3389/fimmu.2018.03151. eCollection 2018. Front Immunol. 2019. PMID: 30697213 Free PMC article. Review. - Innate immune function of the neisserial porins and the relationship to vaccine adjuvant activity.
Wetzler LM. Wetzler LM. Future Microbiol. 2010 May;5(5):749-58. doi: 10.2217/fmb.10.41. Future Microbiol. 2010. PMID: 20441547 Free PMC article. Review.
Cited by
- Evidence of horizontal gene transfer within porB in 19 018 whole-genome Neisseria spp. isolates: a global phylogenetic analysis.
Manoharan-Basil SS, Gestels Z, Abdellati S, Akomoneh EA, Kenyon C. Manoharan-Basil SS, et al. Microb Genom. 2023 Jun;9(6):mgen001041. doi: 10.1099/mgen.0.001041. Microb Genom. 2023. PMID: 37294009 Free PMC article. - Evolutionary and structural aspects of Solanaceae RNases T2.
Thompson CE, Brisolara-Corrêa L, Thompson HN, Stassen H, Freitas LB. Thompson CE, et al. Genet Mol Biol. 2022 Dec 16;46(1 Suppl 1):e20220115. doi: 10.1590/1678-4685-GMB-2022-0115. eCollection 2022. Genet Mol Biol. 2022. PMID: 36534953 Free PMC article. - Pathogenic Neisseria Bind the Complement Protein CFHR5 via Outer Membrane Porins.
Yee WX, Tang CM, Lavender H. Yee WX, et al. Infect Immun. 2022 Oct 20;90(10):e0037722. doi: 10.1128/iai.00377-22. Epub 2022 Oct 4. Infect Immun. 2022. PMID: 36194022 Free PMC article. - Mosaic Evolution of Beta-Barrel-Porin-Encoding Genes in Escherichia coli.
Chen X, Cai X, Chen Z, Wu J, Hao G, Luo Q, Liu S, Zhang J, Hu Y, Zhu G, Koester W, White AP, Cai Y, Wang Y. Chen X, et al. Appl Environ Microbiol. 2022 Apr 12;88(7):e0006022. doi: 10.1128/aem.00060-22. Epub 2022 Mar 14. Appl Environ Microbiol. 2022. PMID: 35285711 Free PMC article. - GenomegaMap: Within-Species Genome-Wide dN/dS Estimation from over 10,000 Genomes.
Wilson DJ; CRyPTIC Consortium. Wilson DJ, et al. Mol Biol Evol. 2020 Aug 1;37(8):2450-2460. doi: 10.1093/molbev/msaa069. Mol Biol Evol. 2020. PMID: 32167543 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources