Role of histone H3 lysine 27 methylation in Polycomb-group silencing - PubMed (original) (raw)

. 2002 Nov 1;298(5595):1039-43.

doi: 10.1126/science.1076997. Epub 2002 Sep 26.

Affiliations

• PMID: 12351676
• DOI: 10.1126/science.1076997

Role of histone H3 lysine 27 methylation in Polycomb-group silencing

Ru Cao et al. Science. 2002.

Abstract

Polycomb group (PcG) proteins play important roles in maintaining the silent state of HOX genes. Recent studies have implicated histone methylation in long-term gene silencing. However, a connection between PcG-mediated gene silencing and histone methylation has not been established. Here we report the purification and characterization of an EED-EZH2 complex, the human counterpart of the Drosophila ESC-E(Z) complex. We demonstrate that the complex specifically methylates nucleosomal histone H3 at lysine 27 (H3-K27). Using chromatin immunoprecipitation assays, we show that H3-K27 methylation colocalizes with, and is dependent on, E(Z) binding at an Ultrabithorax (Ubx) Polycomb response element (PRE), and that this methylation correlates with Ubx repression. Methylation on H3-K27 facilitates binding of Polycomb (PC), a component of the PRC1 complex, to histone H3 amino-terminal tail. Thus, these studies establish a link between histone methylation and PcG-mediated gene silencing.

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