Molecular quantitation of thymic output in mice and the effect of IL-7 - PubMed (original) (raw)
Molecular quantitation of thymic output in mice and the effect of IL-7
Jeffrey Pido-Lopez et al. Eur J Immunol. 2002 Oct.
Free article
Abstract
Aging of the murine thymus is accompanied by a measurable loss of weight and cellularity and a marked reduction in its output of T lymphocytes. This study employs a molecular approach to determine changes in the output of the murine thymus using a novel assay system based on the detection and quantitation of the excised TCRdelta DNA locus from within the TCRalpha chain genes. In alpha beta(+) T cells such delta excision circles (deltaEC) are present at higher levels in naive T cells as compared with memory T cell populations, are non-replicating, and diluted within the total peripheral alpha beta(+) T cell pool with advancing age. This assay permits the assessment of thymic output in older animals where previous analysis was hampered by the transient nature of the naive T cell surface phenotype, and so allows the assessment of the efficacy of IL-7 as an agent to reverse thymic atrophy. Treatment of old mice with IL-7 although producing no overall change in the total number of alpha beta(+) T cells in the peripheral T cell pool altered the component subsets. Mice treated with IL-7 showed increases in the number of alpha beta(+)TCR cells possessing deltaEC commensurate with improved thymic output, and the splenic T cells from IL-7-treated mice performed significantly better in in vitro functional assays compared to those from age-matched saline-treated controls.
Similar articles
- Thymic output and functionality of the IL-7/IL-7 receptor system in centenarians: implications for the neolymphogenesis at the limit of human life.
Nasi M, Troiano L, Lugli E, Pinti M, Ferraresi R, Monterastelli E, Mussi C, Salvioli G, Franceschi C, Cossarizza A. Nasi M, et al. Aging Cell. 2006 Apr;5(2):167-75. doi: 10.1111/j.1474-9726.2006.00204.x. Aging Cell. 2006. PMID: 16626395 - T cell receptor excision circle assessment of thymopoiesis in aging mice.
Sempowski GD, Gooding ME, Liao HX, Le PT, Haynes BF. Sempowski GD, et al. Mol Immunol. 2002 Mar;38(11):841-8. doi: 10.1016/s0161-5890(01)00122-5. Mol Immunol. 2002. PMID: 11922942 - T cell development, ageing and Interleukin-7.
Aspinall R. Aspinall R. Mech Ageing Dev. 2006 Jun;127(6):572-8. doi: 10.1016/j.mad.2006.01.016. Epub 2006 Mar 10. Mech Ageing Dev. 2006. PMID: 16529797 Review. - Reversal of thymic atrophy.
Henson SM, Pido-Lopez J, Aspinall R. Henson SM, et al. Exp Gerontol. 2004 Apr;39(4):673-8. doi: 10.1016/j.exger.2003.10.030. Exp Gerontol. 2004. PMID: 15050305 Review. - T cells developing in fetal thymus of T-cell receptor alpha-chain transgenic mice colonize gammadelta T-cell-specific epithelial niches but lack long-term reconstituting potential.
Leandersson K, Jaensson E, Ivars F. Leandersson K, et al. Immunology. 2006 Sep;119(1):134-42. doi: 10.1111/j.1365-2567.2006.02415.x. Immunology. 2006. PMID: 16925528 Free PMC article.
Cited by
- Age-related thymic involution: Mechanisms and functional impact.
Liang Z, Dong X, Zhang Z, Zhang Q, Zhao Y. Liang Z, et al. Aging Cell. 2022 Aug;21(8):e13671. doi: 10.1111/acel.13671. Epub 2022 Jul 12. Aging Cell. 2022. PMID: 35822239 Free PMC article. Review. - Major features of immunesenescence, including reduced thymic output, are ameliorated by high levels of physical activity in adulthood.
Duggal NA, Pollock RD, Lazarus NR, Harridge S, Lord JM. Duggal NA, et al. Aging Cell. 2018 Apr;17(2):e12750. doi: 10.1111/acel.12750. Epub 2018 Mar 8. Aging Cell. 2018. PMID: 29517845 Free PMC article. - Effect of IL-7 Therapy on Naive and Memory T Cell Homeostasis in Aged Rhesus Macaques.
Okoye AA, Rohankhedkar M, Konfe AL, Abana CO, Reyes MD, Clock JA, Duell DM, Sylwester AW, Sammader P, Legasse AW, Park BS, Axthelm MK, Nikolich-Žugich J, Picker LJ. Okoye AA, et al. J Immunol. 2015 Nov 1;195(9):4292-305. doi: 10.4049/jimmunol.1500609. Epub 2015 Sep 28. J Immunol. 2015. PMID: 26416281 Free PMC article. - Insights into thymic involution in tumor-bearing mice.
Carrio R, Lopez DM. Carrio R, et al. Immunol Res. 2013 Dec;57(1-3):106-14. doi: 10.1007/s12026-013-8446-3. Immunol Res. 2013. PMID: 24198066 Review. - The effect of age on thymic function.
Palmer DB. Palmer DB. Front Immunol. 2013 Oct 7;4:316. doi: 10.3389/fimmu.2013.00316. Front Immunol. 2013. PMID: 24109481 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical