Enhancement of somatomedin titers of normal and hypopituitary sera by addition of L-triiodothyronone in vitro at physiological concentrations - PubMed (original) (raw)
Enhancement of somatomedin titers of normal and hypopituitary sera by addition of L-triiodothyronone in vitro at physiological concentrations
T K Audhya et al. Proc Natl Acad Sci U S A. 1975 Feb.
Abstract
Somatomedin potencies of sera were assayed by following sulfation of mucopolysaccharides in chick embryo sterna in vitro. Apparent potencies of sera from hypophysectomized rats, maintained on a low-iodine diet, were restored to levels above normal by addition to the incubation medium of L-triiodothyronine at 10-7 mol/liter of serum. The potencies of normal rat, human, and fetal calf sera were raised 1.3- to 3-fold by addition of triiodothyronine at 10-9-10-7 mol/liter of serum. L-Thyroxine was about 10 times less effective than triiodothyronine. Triiodothyronine alone did not stimulate sulfation to nearly the same extent as triiodothyronine plus serum, even at higher concentrations. Serum could not be substituted in this system by any of six purified hormones, nor by trace metals or amino acids not included in the incubation mixture. It is concluded that triiodothyronine, in combination with a factor in serum, causes a rapid stimulation of sulfation in chick embryo cartilage, and that thyroid hormones may be involved in the action of normal serum on this tissue.
Similar articles
- Nonsuppressible insulin-like activity and thyroid hormones: major pituitary-dependent sulfation factors for chick embryo cartilage.
Froesch ER, Zapf J, Audhya TK, Ben-Porath E, Segen BJ, Gibson KD. Froesch ER, et al. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2904-8. doi: 10.1073/pnas.73.8.2904. Proc Natl Acad Sci U S A. 1976. PMID: 1066701 Free PMC article. - Serum somatomedin stimulation in thyroxine-treated hypophysectomized rats.
Gaspard T, Wondergem R, Hamamdzić M, Klitgaard HM. Gaspard T, et al. Endocrinology. 1978 Feb;102(2):606-11. doi: 10.1210/endo-102-2-606. Endocrinology. 1978. PMID: 743982 - Effects of medium composition and metabolic inhibitors on glycosaminoglycan synthesis in chick embryo cartilage and its stimulation by serum and triiodothyronine.
Audhya TK, Gibson KD. Audhya TK, et al. Biochim Biophys Acta. 1976 Jul 21;437(2):364-76. doi: 10.1016/0304-4165(76)90006-4. Biochim Biophys Acta. 1976. PMID: 133721 - Cartilage sulfation and serum somatomedin in rats during and after cortisone-induced growth arrest.
Mosier HD Jr, Jansons RA, Hill RR, Dearden LC. Mosier HD Jr, et al. Endocrinology. 1976 Aug;99(2):580-9. doi: 10.1210/endo-99-2-580. Endocrinology. 1976. PMID: 954655 - Steroid hormone effects on sonatomedin. I. Somatomedin action in vitro.
Phillips LS, Herington AC, Daughaday WH. Phillips LS, et al. Endocrinology. 1975 Oct;97(4):780-6. doi: 10.1210/endo-97-4-780. Endocrinology. 1975. PMID: 1193005
Cited by
- Triiodothyronine stimulates maturation of porcine growth-plate cartilage in vitro.
Burch WM, Lebovitz HE. Burch WM, et al. J Clin Invest. 1982 Sep;70(3):496-504. doi: 10.1172/jci110641. J Clin Invest. 1982. PMID: 7107892 Free PMC article. - The mode of action of 4-methylumbelliferyl beta-D-xyloside on the synthesis of chondroitin sulphate in embryonic-chicken sternum.
Gibson KD, Segen BJ. Gibson KD, et al. Biochem J. 1977 Oct 15;168(1):65-79. doi: 10.1042/bj1680065. Biochem J. 1977. PMID: 597264 Free PMC article. - Insulin-Like Growth Factor Pathway and the Thyroid.
Smith TJ. Smith TJ. Front Endocrinol (Lausanne). 2021 Jun 4;12:653627. doi: 10.3389/fendo.2021.653627. eCollection 2021. Front Endocrinol (Lausanne). 2021. PMID: 34149612 Free PMC article. Review. - The effect of beta-D-xylosides on chondroitin sulphate biosynthesis in embryonic chicken cartilage in the absence of protein synthesis inhibitors.
Gibson KD, Segen BJ, Audhya TK. Gibson KD, et al. Biochem J. 1977 Feb 15;162(2):217-33. doi: 10.1042/bj1620217. Biochem J. 1977. PMID: 849282 Free PMC article. - Nonsuppressible insulin-like activity and thyroid hormones: major pituitary-dependent sulfation factors for chick embryo cartilage.
Froesch ER, Zapf J, Audhya TK, Ben-Porath E, Segen BJ, Gibson KD. Froesch ER, et al. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2904-8. doi: 10.1073/pnas.73.8.2904. Proc Natl Acad Sci U S A. 1976. PMID: 1066701 Free PMC article.
References
- Physiol Rev. 1962 Oct;42:605-73 - PubMed
- J Clin Endocrinol Metab. 1963 Jul;23:638-50 - PubMed
- J Clin Endocrinol Metab. 1964 Aug;24:785-93 - PubMed
- Endocrinology. 1974 Dec;95(6):1614-20 - PubMed
- Endocrinology. 1973 Jul;93(1):61-73 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources