Nitric oxide inhibits ADP-ribosyl cyclase through a cGMP-independent pathway in airway smooth muscle - PubMed (original) (raw)
. 2002 Nov;283(5):L1065-71.
doi: 10.1152/ajplung.00064.2002.
Affiliations
- PMID: 12376359
- DOI: 10.1152/ajplung.00064.2002
Free article
Nitric oxide inhibits ADP-ribosyl cyclase through a cGMP-independent pathway in airway smooth muscle
Thomas A White et al. Am J Physiol Lung Cell Mol Physiol. 2002 Nov.
Free article
Abstract
There is evidence for a role of cyclic ADP-ribose (cADPR) in intracellular Ca2+ regulation in smooth muscle. cADPR is synthesized and degraded by ADP-ribosyl cyclase and cADPR hydrolase, respectively, by a bifunctional protein, CD38. Nitric oxide (NO) inhibits intracellular Ca2+ mobilization in airway smooth muscle. The present study was designed to determine whether this inhibition is due to regulation of ADP-ribosyl cyclase and/or cADPR hydrolase activity. Sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine, NO donors, produced a concentration-dependent decrease in ADP-ribosyl cyclase, but not cADPR hydrolase, activity. The NO scavenger carboxy-PTIO prevented and reversed, and reduced glutathione prevented, the inhibition of ADP-ribosyl cyclase by SNP, suggesting S-nitrosylation by NO as a mechanism. N-ethylmaleimide, which covalently modifies protein sulfhydryl groups, making them incapable of nitrosylation, produced a marked inhibition of ADP-ribosyl cyclase, but not cADPR hydrolase, activity. SNP and N-ethylmaleimide significantly inhibited the ADP-ribosyl cyclase activity in recombinant human CD38 without affecting the cADPR hydrolase activity. These results provide a novel mechanism for differential regulation of CD38 by NO through a cGMP-independent pathway involving S-nitrosylation of thiols.
Similar articles
- Subcellular localization of cyclic ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activities in porcine airway smooth muscle.
White TA, Johnson S, Walseth TF, Lee HC, Graeff RM, Munshi CB, Prakash YS, Sieck GC, Kannan MS. White TA, et al. Biochim Biophys Acta. 2000 Oct 20;1498(1):64-71. doi: 10.1016/s0167-4889(00)00077-x. Biochim Biophys Acta. 2000. PMID: 11042351 - Changes in CD38 expression and ADP-ribosyl cyclase activity in rat myometrium during pregnancy: influence of sex steroid hormones.
Dogan S, Deshpande DA, Kannan MS, Walseth TF. Dogan S, et al. Biol Reprod. 2004 Jul;71(1):97-103. doi: 10.1095/biolreprod.103.026245. Epub 2004 Mar 3. Biol Reprod. 2004. PMID: 14998907 - Extracellular cyclic ADP-ribose potentiates ACh-induced contraction in bovine tracheal smooth muscle.
Franco L, Bruzzone S, Song P, Guida L, Zocchi E, Walseth TF, Crimi E, Usai C, De Flora A, Brusasco V. Franco L, et al. Am J Physiol Lung Cell Mol Physiol. 2001 Jan;280(1):L98-L106. doi: 10.1152/ajplung.2001.280.1.L98. Am J Physiol Lung Cell Mol Physiol. 2001. PMID: 11133499 - Cyclic ADP-ribose as a potential second messenger for neuronal Ca2+ signaling.
Higashida H, Hashii M, Yokoyama S, Hoshi N, Asai K, Kato T. Higashida H, et al. J Neurochem. 2001 Jan;76(2):321-31. doi: 10.1046/j.1471-4159.2001.00082.x. J Neurochem. 2001. PMID: 11208895 Review. - Cyclic ADP-ribose as a second messenger revisited from a new aspect of signal transduction from receptors to ADP-ribosyl cyclase.
Higashida H, Hashii M, Yokoyama S, Hoshi N, Chen XL, Egorova A, Noda M, Zhang JS. Higashida H, et al. Pharmacol Ther. 2001 May-Jun;90(2-3):283-96. doi: 10.1016/s0163-7258(01)00142-5. Pharmacol Ther. 2001. PMID: 11578661 Review.
Cited by
- Vascular physiology of a Ca2+ mobilizing second messenger - cyclic ADP-ribose.
Zhang AY, Li PL. Zhang AY, et al. J Cell Mol Med. 2006 Apr-Jun;10(2):407-22. doi: 10.1111/j.1582-4934.2006.tb00408.x. J Cell Mol Med. 2006. PMID: 16796808 Free PMC article. Review. - Multiple signal transduction pathways mediated by 5-HT receptors.
Noda M, Higashida H, Aoki S, Wada K. Noda M, et al. Mol Neurobiol. 2004 Feb;29(1):31-9. doi: 10.1385/MN:29:1:31. Mol Neurobiol. 2004. PMID: 15034221 Review. - Nitric oxide metabolism in asthma pathophysiology.
Ghosh S, Erzurum SC. Ghosh S, et al. Biochim Biophys Acta. 2011 Nov;1810(11):1008-16. doi: 10.1016/j.bbagen.2011.06.009. Epub 2011 Jun 21. Biochim Biophys Acta. 2011. PMID: 21718755 Free PMC article. Review. - Nitric oxide induces airway smooth muscle cell relaxation by decreasing the frequency of agonist-induced Ca2+ oscillations.
Perez-Zoghbi JF, Bai Y, Sanderson MJ. Perez-Zoghbi JF, et al. J Gen Physiol. 2010 Mar;135(3):247-59. doi: 10.1085/jgp.200910365. J Gen Physiol. 2010. PMID: 20176853 Free PMC article. - S-nitrosoglutathione supplementation to ovalbumin-sensitized and -challenged mice ameliorates methacholine-induced bronchoconstriction.
Foster MW, Yang Z, Potts EN, Michael Foster W, Que LG. Foster MW, et al. Am J Physiol Lung Cell Mol Physiol. 2011 Nov;301(5):L739-44. doi: 10.1152/ajplung.00134.2011. Epub 2011 Jul 22. Am J Physiol Lung Cell Mol Physiol. 2011. PMID: 21784966 Free PMC article. Retracted.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous