Embryonic intermediate filament, nestin, expression following traumatic spinal cord injury in adult rats - PubMed (original) (raw)
Embryonic intermediate filament, nestin, expression following traumatic spinal cord injury in adult rats
S Shibuya et al. Neuroscience. 2002.
Abstract
Precursor cells in the ependyma of the lateral ventricles of adult mammalian brain have been reported in brain, and also in the spinal cord. The present study used antibody to the intermediate filament protein (nestin) as an immunohistochemical marker for neural stem cells and precursor cells in a rat model of spinal cord trauma. Male Sprague-Dawley rats (n=25) had a laminectomy at Thll-Thl2, and spinal cord contusion was created by compression with 30 g of force for 10 min. The rats were killed at 24 h, 1 week and 4 weeks after injury, and four levels of the spinal cord were examined: 5 mm and 10 mm, both rostral and caudal region to the injury center. Time- and region-dependent alterations of nestin immunoreactivity were analyzed. Revealed at 24 h post-injury, 5 mm rostral and caudal to the lesions, nestin expression was observed in ependymal cells and around the hemorrhagic and necrotic lesion located in dorsal spinal cord, peaking at 1 week after injury. Moreover, nestin expression was also observed in the white matter of ventral spinal cord, extending into arborizing processes centripetally from the pial surface toward the central canal. At 4 weeks after injury, nestin expression in ependyma decreased 10 mm from the injury site. But nestin expression in white matter increased dramatically with a 100-fold increase in nestin originating from the pial surface, and extension now to all the white matter. The latter was accompanied by glial fibrillary acidic protein positivity into very long arborizing processes, morphologically compatible with radial glia. The findings suggest two possible sources of precursor cells in adult mammalian spinal cord; ependyma of the central canal and subpial astrocytes. Subpial astrocytes may be associated with neural repair and regeneration after spinal cord injury.
Similar articles
- Temporal progressive antigen expression in radial glia after contusive spinal cord injury in adult rats.
Shibuya S, Miyamoto O, Itano T, Mori S, Norimatsu H. Shibuya S, et al. Glia. 2003 Apr 15;42(2):172-83. doi: 10.1002/glia.10203. Glia. 2003. PMID: 12655601 - Cell proliferation and nestin expression in the ependyma of the adult rat spinal cord after injury.
Namiki J, Tator CH. Namiki J, et al. J Neuropathol Exp Neurol. 1999 May;58(5):489-98. doi: 10.1097/00005072-199905000-00008. J Neuropathol Exp Neurol. 1999. PMID: 10331437 - [Reactive astrocytes and nestin expression in adult rats following spinal cord compression injury].
Yang PL, He XJ, Li HP, Lan BS, Wang D, Wang GY, Xu SY, Liu YH. Yang PL, et al. Nan Fang Yi Ke Da Xue Xue Bao. 2008 Oct;28(10):1752-5. Nan Fang Yi Ke Da Xue Xue Bao. 2008. PMID: 18971163 Chinese. - The activation of dormant ependymal cells following spinal cord injury.
Rodriguez-Jimenez FJ, Jendelova P, Erceg S. Rodriguez-Jimenez FJ, et al. Stem Cell Res Ther. 2023 Jul 5;14(1):175. doi: 10.1186/s13287-023-03395-4. Stem Cell Res Ther. 2023. PMID: 37408068 Free PMC article. Review. - The spinal cord ependymal region: a stem cell niche in the caudal central nervous system.
Hugnot JP, Franzen R. Hugnot JP, et al. Front Biosci (Landmark Ed). 2011 Jan 1;16(3):1044-59. doi: 10.2741/3734. Front Biosci (Landmark Ed). 2011. PMID: 21196217 Review.
Cited by
- Cortical Pathology in Vanishing White Matter.
Man JHK, van Gelder CAGH, Breur M, Okkes D, Molenaar D, van der Sluis S, Abbink T, Altelaar M, van der Knaap MS, Bugiani M. Man JHK, et al. Cells. 2022 Nov 12;11(22):3581. doi: 10.3390/cells11223581. Cells. 2022. PMID: 36429009 Free PMC article. - Lineage tracing reveals the origin of Nestin-positive cells are heterogeneous and rarely from ependymal cells after spinal cord injury.
Xue X, Shu M, Xiao Z, Zhao Y, Li X, Zhang H, Fan Y, Wu X, Chen B, Xu B, Yang Y, Liu W, Liu S, Dai J. Xue X, et al. Sci China Life Sci. 2022 Apr;65(4):757-769. doi: 10.1007/s11427-020-1901-4. Epub 2021 Mar 24. Sci China Life Sci. 2022. PMID: 33772745 - Improvement of Rat Spinal Cord Injury Following Lentiviral Vector-Transduced Neural Stem/Progenitor Cells Derived from Human Epileptic Brain Tissue Transplantation with a Self-assembling Peptide Scaffold.
Abdolahi S, Aligholi H, Khodakaram-Tafti A, Khaleghi Ghadiri M, Stummer W, Gorji A. Abdolahi S, et al. Mol Neurobiol. 2021 Jun;58(6):2481-2493. doi: 10.1007/s12035-020-02279-5. Epub 2021 Jan 14. Mol Neurobiol. 2021. PMID: 33443682 Free PMC article. - Curcumin Increase the Expression of Neural Stem/Progenitor Cells and Improves Functional Recovery after Spinal Cord Injury.
Bang WS, Kim KT, Seo YJ, Cho DC, Sung JK, Kim CH. Bang WS, et al. J Korean Neurosurg Soc. 2018 Jan;61(1):10-18. doi: 10.3340/jkns.2017.0203.003. Epub 2017 Dec 29. J Korean Neurosurg Soc. 2018. PMID: 29354231 Free PMC article. - Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury?
Salem N, Salem MY, Elmaghrabi MM, Elawady MA, Elawady MA, Sabry D, Shamaa A, Elkasapy AH, Ibrhim N, Elamir A. Salem N, et al. Neural Regen Res. 2017 Dec;12(12):2050-2058. doi: 10.4103/1673-5374.221163. Neural Regen Res. 2017. PMID: 29323045 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical