A multiplex polymerase chain reaction-based diagnostic method for bacterial vaginosis - PubMed (original) (raw)
A multiplex polymerase chain reaction-based diagnostic method for bacterial vaginosis
Mana Obata-Yasuoka et al. Obstet Gynecol. 2002 Oct.
Abstract
Objective: To develop a polymerase chain reaction (PCR)-based diagnostic method for bacterial vaginosis using bacterial vaginosis-associated anaerobes.
Methods: A multiple PCR assay was developed using primers specific to 16S ribosomal deoxyribonucleic acid (DNA) (Mobiluncus mulieris and Mobiluncus curtisii), nanH (Bacteroides fragilis), and an internal spacer region of ribosomal DNA (Gardnerella vaginalis). The vaginal swabs from pregnant and nonpregnant women were examined by Gram stain-based Nugent scoring system. One hundred seventy-two samples of 853 Gram stain-interpretable samples were randomly selected and subjected to multiplex PCR assay.
Results: The sensitivity of the PCR assay ranged from 10 to 10 colony-forming units per vaginal swab. The prevalence of the bacterial vaginosis, intermediate, and normal categories was found by Nugent scoring system to be 21.6% (184/853), 26.0% (222/853), and 52.4% (447/853), respectively. By the multiplex PCR-based diagnostic method, 20.3% (35/172) of the samples were identified as bacterial vaginosis. The diagnostic sensitivity, specificity, positive predictive value, and negative predictive value of multiplex PCR in comparison with Gram stain examination were 78.4% (95% confidence interval [CI] 65.1%, 91.6%), 95.6% (95% CI 92.1%, 99.0%), 82.9% (95% CI 70.4%, 95.4%), and 94.2% (95% CI 90.3%, 98.1%), respectively.
Conclusion: This multiplex PCR can be used as a diagnostic or screening test for bacterial vaginosis.
Similar articles
- [Value of the score for Gram strains in the diagnosis of bacterial vaginosis].
Nicand E, Cavallo JD, Crenn Y, Meyran M. Nicand E, et al. Pathol Biol (Paris). 1994 May;42(5):539-43. Pathol Biol (Paris). 1994. PMID: 7824329 French. - Molecular quantification of Gardnerella vaginalis and Atopobium vaginae loads to predict bacterial vaginosis.
Menard JP, Fenollar F, Henry M, Bretelle F, Raoult D. Menard JP, et al. Clin Infect Dis. 2008 Jul 1;47(1):33-43. doi: 10.1086/588661. Clin Infect Dis. 2008. PMID: 18513147 - Prevalence of Mobiluncus spp among women with and without bacterial vaginosis as detected by polymerase chain reaction.
Schwebke JR, Lawing LF. Schwebke JR, et al. Sex Transm Dis. 2001 Apr;28(4):195-9. doi: 10.1097/00007435-200104000-00002. Sex Transm Dis. 2001. PMID: 11318249 - Diagnostic microbiology of bacterial vaginosis.
Hillier SL. Hillier SL. Am J Obstet Gynecol. 1993 Aug;169(2 Pt 2):455-9. doi: 10.1016/0002-9378(93)90340-o. Am J Obstet Gynecol. 1993. PMID: 8357044 Review. - Bacterial vaginosis a "broad overview".
González Pedraza Avilés A, Ortíz Zaragoza MC, Irigoyen Coria A. González Pedraza Avilés A, et al. Rev Latinoam Microbiol. 1999 Jan-Mar;41(1):25-34. Rev Latinoam Microbiol. 1999. PMID: 10932748 Review.
Cited by
- Sensitive Detection of Thirteen Bacterial Vaginosis-Associated Agents Using Multiplex Polymerase Chain Reaction.
Malaguti N, Bahls LD, Uchimura NS, Gimenes F, Consolaro ME. Malaguti N, et al. Biomed Res Int. 2015;2015:645853. doi: 10.1155/2015/645853. Epub 2015 May 20. Biomed Res Int. 2015. PMID: 26078959 Free PMC article. - Vaginal Dysbiosis and Partial Bacterial Vaginosis: The Interpretation of the "Grey Zones" of Clinical Practice.
Campisciano G, Zanotta N, Petix V, Giangreco M, Ricci G, Maso G, Comar M, De Seta F. Campisciano G, et al. Diagnostics (Basel). 2021 Jan 28;11(2):191. doi: 10.3390/diagnostics11020191. Diagnostics (Basel). 2021. PMID: 33525708 Free PMC article. - Comparison between Gram stain and culture for the characterization of vaginal microflora: definition of a distinct grade that resembles grade I microflora and revised categorization of grade I microflora.
Verhelst R, Verstraelen H, Claeys G, Verschraegen G, Van Simaey L, De Ganck C, De Backer E, Temmerman M, Vaneechoutte M. Verhelst R, et al. BMC Microbiol. 2005 Oct 14;5:61. doi: 10.1186/1471-2180-5-61. BMC Microbiol. 2005. PMID: 16225680 Free PMC article. - The laboratory diagnosis of bacterial vaginosis.
Money D. Money D. Can J Infect Dis Med Microbiol. 2005 Mar;16(2):77-9. doi: 10.1155/2005/230319. Can J Infect Dis Med Microbiol. 2005. PMID: 18159532 Free PMC article. - Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis.
Verhelst R, Verstraelen H, Claeys G, Verschraegen G, Delanghe J, Van Simaey L, De Ganck C, Temmerman M, Vaneechoutte M. Verhelst R, et al. BMC Microbiol. 2004 Apr 21;4:16. doi: 10.1186/1471-2180-4-16. BMC Microbiol. 2004. PMID: 15102329 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources