Transgenic mice with green fluorescent protein-labeled pancreatic beta -cells - PubMed (original) (raw)
. 2003 Jan;284(1):E177-83.
doi: 10.1152/ajpendo.00321.2002. Epub 2002 Sep 17.
Affiliations
- PMID: 12388130
- DOI: 10.1152/ajpendo.00321.2002
Free article
Transgenic mice with green fluorescent protein-labeled pancreatic beta -cells
Manami Hara et al. Am J Physiol Endocrinol Metab. 2003 Jan.
Free article
Abstract
We have generated transgenic mice that express green fluorescent protein (GFP) under the control of the mouse insulin I gene promoter (MIP). The MIP-GFP mice develop normally and are indistinguishable from control animals with respect to glucose tolerance and pancreatic insulin content. Histological studies showed that the MIP-GFP mice had normal islet architecture with coexpression of insulin and GFP in the beta-cells of all islets. We observed GFP expression in islets from embryonic day E13.5 through adulthood. Studies of beta-cell function revealed no difference in glucose-induced intracellular calcium mobilization between islets from transgenic and control animals. We prepared single-cell suspensions from both isolated islets and whole pancreas from MIP-GFP-transgenic mice and sorted the beta-cells by fluorescence-activated cell sorting based on their green fluorescence. These studies showed that 2.4 +/- 0.2% (n = 6) of the cells in the pancreas of newborn (P1) and 0.9 +/- 0.1% (n = 5) of 8-wk-old mice were beta-cells. The MIP-GFP-transgenic mouse may be a useful tool for studying beta-cell biology in normal and diabetic animals.
Similar articles
- Targeting of a phogrin-green fluorescent protein chimaera to insulin secretory granules of pancreatic beta-cells in transgenic mice.
Bright NA, Walters J, Wasmeier C, Hutton JC. Bright NA, et al. Diabetes Metab. 2002 Dec;28(6 Pt 2):3S29-36; discussion 3S108-12. Diabetes Metab. 2002. PMID: 12688631 - Imaging pancreatic beta-cells in the intact pancreas.
Hara M, Dizon RF, Glick BS, Lee CS, Kaestner KH, Piston DW, Bindokas VP. Hara M, et al. Am J Physiol Endocrinol Metab. 2006 May;290(5):E1041-7. doi: 10.1152/ajpendo.00365.2005. Epub 2005 Dec 20. Am J Physiol Endocrinol Metab. 2006. PMID: 16368785 - Beta cells within single human islets originate from multiple progenitors.
Scharfmann R, Xiao X, Heimberg H, Mallet J, Ravassard P. Scharfmann R, et al. PLoS One. 2008;3(10):e3559. doi: 10.1371/journal.pone.0003559. Epub 2008 Oct 29. PLoS One. 2008. PMID: 18958289 Free PMC article. - Expression of human insulin and hybrid insulin genes in pancreatic beta cells of transgenic mice.
Bucchini D, Deltour L, Jami J. Bucchini D, et al. Horm Res. 1989;32(1-3):47-52. doi: 10.1159/000181243. Horm Res. 1989. PMID: 2693315 Review. - Seeing is believing: how the MIP-luc mouse can advance the field of islet transplantation and β-cell regeneration.
Grossman E, Tao J, Wilson R, Park SY, Bell G, Chong A. Grossman E, et al. Islets. 2010 Jul-Aug;2(4):261-2. doi: 10.4161/isl.2.4.12028. Islets. 2010. PMID: 21099322 Review.
Cited by
- Engineered extracellular vesicles from human skin cells induce pro-β-cell conversions in pancreatic ductal cells.
Ortega-Pineda L, Guilfoyle E, Rincon-Benavides MA, Anaparthi AL, Lemmerman LR, Cuellar-Gaviria TZ, Lawrence W, Buss JL, Deng B, Blackstone BN, Salazar-Puerta A, McComb DW, Powell H, Gallego-Perez D, Higuita-Castro N. Ortega-Pineda L, et al. Adv Nanobiomed Res. 2023 Oct;3(10):2200173. doi: 10.1002/anbr.202200173. Epub 2023 Sep 5. Adv Nanobiomed Res. 2023. PMID: 38911285 Free PMC article. - The adhesion GPCR GPR116/ADGRF5 has a dual function in pancreatic islets regulating somatostatin release and islet development.
Röthe J, Kraft R, Ricken A, Kaczmarek I, Matz-Soja M, Winter K, Dietzsch AN, Buchold J, Ludwig MG, Liebscher I, Schöneberg T, Thor D. Röthe J, et al. Commun Biol. 2024 Jan 16;7(1):104. doi: 10.1038/s42003-024-05783-9. Commun Biol. 2024. PMID: 38228886 Free PMC article. - Local Dialogues Between the Endocrine and Exocrine Cells in the Pancreas.
Slak Rupnik M, Hara M. Slak Rupnik M, et al. Diabetes. 2024 Apr 1;73(4):533-541. doi: 10.2337/db23-0760. Diabetes. 2024. PMID: 38215069 Review. - The anterior chamber of the eye technology and its anatomical, optical, and immunological bases.
Yang SN, Shi Y, Berggren PO. Yang SN, et al. Physiol Rev. 2024 Jul 1;104(3):881-929. doi: 10.1152/physrev.00024.2023. Epub 2024 Jan 11. Physiol Rev. 2024. PMID: 38206586 Free PMC article. Review. - Pancreatic islet cell type-specific transcriptomic changes during pregnancy and postpartum.
Chung JY, Ma Y, Zhang D, Bickerton HH, Stokes E, Patel SB, Tse HM, Feduska J, Welner RS, Banerjee RR. Chung JY, et al. iScience. 2023 Mar 17;26(4):106439. doi: 10.1016/j.isci.2023.106439. eCollection 2023 Apr 21. iScience. 2023. PMID: 37020962 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases